Effects of EGFL7-blocking Monoclone Antibody Inhibiting Proliferation and Invasion of Mouse Adrenocorticotropic Hormone Pituitary Adenoma AtT-20 Cells in vitro

Abstract

Abstract: Objective To study the effects of EGFL7-blocking monoclone antibody inhibiting the proliferation and invasion of adrenocorticotropic hormone (ACTH) pituitary adenoma AtT-20 cells in vitro. Methods After treated with different concentrations of EGFL7-blocking monoclone antibody for 24, 48, 72 h, the proliferation of AtT-20 cells was determined by MTS. Simultaneously, ACTH secretion of AtT-20 cells by ELISA and the mRNA expression of invasion related genes such as E-cadherin, snail, vimentin were detected by Real-time PCR. Results EGFL7-blocking monoclone antibody could effectively inhibit the proliferation and ACTH secretion of AtT-20 cells in a dose-dependent and time-dependent manner. Also, EGFL7-blocking monoclone antibody significant down-regulated the mRNA level of E-cadherin、snail, vimentin in AtT-20 cells. Conclusion EGFL7-blocking monoclone antibody could inhibit mouse pituitary adenoma AtT-20 cells proliferation and invasion.

Key words: EGFL7, monoclone antibody, AtT-20 cells, proliferation, invasion

CLC Number:

R965

LIU Qian WANG Hong-yun LI Dan GONG Lei, ZHANG Ya-zhuo. Effects of EGFL7-blocking Monoclone Antibody Inhibiting Proliferation and Invasion of Mouse Adrenocorticotropic Hormone Pituitary Adenoma AtT-20 Cells in vitro[J]. Chinese Journal of Pharmacovigilance, 2018, 15(6): 321-324.

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