Abstract: Objective To investigate the effect of involvement of Puerariae Lobatae Radix in fermentation of Semen Sojae Praeparatum (SSP) on the anti-osteoporosis activity in vitro by adding Puerariae Lobatae Radix as an auxiliary material for fermentation of SSP. Methods The SPSS22.0 software was used to analyze the pharmacodynamic activities between the Puerariae Lobatae Radix-adding pure culture fermentation group, the pure culture fermentation without Puerariae Lobatae Radix group, the pure culture fermentation with Puerariae Lobatae Radix as the only auxiliary material group, the Pueraria lobata-involving natural fermentation group, the non-Pueraria lobata-involving natural fermentation group, and the Puerariae Lobatae Radix-involving alone natural fermentation group. At the same time, the optimum fermentation time within the first seven days was compared between pure culture fermentation groups. Results It was found that the best anti-osteoporosis condition was at the concentration of 1×10^-4 g·L^-1, SSP fermented with Pueraria for 4 days was the most effective in promoting osteoblast proliferation, and the proliferation rate was 45.8%. Conclusion This study has confirmed that Puerariae Lobatae Radix, when used as an auxiliary material for fermentation, can effectively improve the anti-osteoporosis activity in vitro, and provide new ideas for the fermentation of traditional Chinese medicines.

Key words: Semen Sojae Praeparatum, fermentation, Puerariae Lobatae Radix, in vitro activity, osteoblast, osteoporosis, proliferation rate