Effects of Tangeretin on Proliferation and Apoptosis of Prolactinoma MMQ Cells and Its Mechanism in vitro

Abstract

Abstract: Objective To study the effects of tangeretin inhibiting the proliferation and inducing apoptosis of prolactinoma MMQ cells. Methods After treated with different concentration of tangeretin for 24, 48, 72 h, the proliferation of prolactinoma MMQ cells was determined by MTS. Simultaneously, the apoptosis ratio of MMQ by Annexin V-FITC/PI and the expression of bcl-2 and bax by Western blotting were detected. Results Tangeretin could effectively inhibit the proliferation of MMQ in a dose-dependent and time-dependent manner. At the same time, tangeretin induced apoptosis and down-regulated the level of bcl-2 and up-regulated the level of bax in MMQ cells. Conclusion Tangeretin maybe induced apoptosis and inhibited the proliferation of MMQ through bcl-2/bax signaling pathway.

Key words: tangeretin, prolactinoma, apoptosis, proliferation

CLC Number:

R965

ZHENG Shu-rong, GAO Hua, LI Dan, WANG Hong-yun, Gong Lei, LIU Qian. Effects of Tangeretin on Proliferation and Apoptosis of Prolactinoma MMQ Cells and Its Mechanism in vitro[J]. Chinese Journal of Pharmacovigilance, 2018, 15(3): 129-131.

References

[1] 中国垂体腺瘤协作组.中国垂体催乳素腺瘤诊治共识（2014版）[J].中华医学杂志,2014,94(31):2406-2411.
[2] 季立津.泌乳素瘤发病机制研究进展[J].医学综述,2016,22(1):55-59.
[3] 王红云,龚磊,刘春晖,等.雌激素受体1在生长激素腺瘤中的表达及意义[J].中华神经外科杂志,2017,33(6):610-614.
[4] 陶燕铎,曹纬国,刘志勤,等.黄酮类化合物药理作用的研究进展[J].西北植物学报,2003,23(12):2241-2247.
[5] Arivazhagan L, Sorimuthu P S. Tangeretin, a citrus pentamethoxyflav-
one, exerts cytostatic effect via p53/ p21 up-regulation and suppresses metastasis in 7,12-dimethylbenz(alpha)anthracene-induced rat mammary carcinoma[J]. J Nutr Biochem,2014,25(11):1140-1153．
[6] 李金艳.橘皮素对人成胶质细胞瘤细胞增殖及凋亡的影响[J].中国老年学杂志,2017,37(12):2891-2893.
[7] 杨连君,曹雪涛,于益芝.bcl-2,bax与肿瘤细胞凋亡[J].中国肿瘤生物治疗杂志,2003,10(3):232-234.
[8] Kang M H, Reynolds C P. Bcl-2 inhibitors: targeting mitochondrial apoptotic pathways in cancer therapy[J]. Clinical Cancer Research, 2009, 15(4):1126-1132.
[9] GillamM P, MolitchM E, Lombardi G, et al. Advances in the treatment of prolactinoma[J]. Endocrine Reviews,2006,27(5):485-534.
[10] 孙青芳,卞留贯,赵卫国,等.垂体泌乳素腺瘤的手术治疗[J].中华神经外科杂志,2006,22(2):109-111.
[11] Shi M D, Liao Y C, Shih Y W, et al. Nobiletin attenuates metastasis via both ERK and P13K/Akt pathways in HGF-treated liver cancer HepG2 cells[J].Phytomedicine, 2013,20(8-9):743-752.
[12] Chen K H, Weng M S, Lin J K. Tangeretin suppresses IL-1 beta-induced eyelooxygenase(COX)-2 expression through inhibition of p38 MAPK, JNK, and AKT activation in human lung carcinoma cells[J]. Biochem Pharmaeol,2007,73(2):215-227.
[13] 托娅,张和平.乳酸杆菌Lactobacillus casei.Zhang的抗肿瘤作用及机制[J].分子诊断与治疗杂志,2010,2(6):394-399.

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