Abstract: Objective To study the effects of Lx2-32c on the proliferation and apoptosis of cervical cell Hela. Methods After treated with different concentration of Lx2-32c for 24, 48, 72 h, the proliferation of cervical cell Hela was determined by MTT. Simultaneously, the apoptosis ratio of Hela by Annexin V-FITC/PI and the expression of Bcl-2 and cleaved-caspase-3 by western blotting were detected. Results Lx2-32c could effectively inhibit the proliferation of Hela in a dose-dependent and time-dependent manner. At the same time, Lx2-32c induced apoptosis and down-regulated the expression of Bcl-2 and up-regulated the expression of cleaved-caspase-3 in Hela. Conclusion Lx2-32c maybe induced apoptosis and inhibited the proliferation of Hela involving in mitochondrial pathway.

Key words: cervical cell Hela, Lx2-32c, apoptosis, proliferation