Abstract: Objective To investigate the protective effects of alcohol extracts from Pteris multifida Poir(EPMP) on cholestatic jaundice induced by α-naphthalene isothiocyanate (ANIT) in mice. Methods ICR mice were given EPMP 125, 250 and 500 mg·kg-1 for 5 days orally, once per day. 2 h after administration of medication on the 3rd day, the mice were given ANIT 80 mg·kg-1 orally once. All of the animals were fasted 12 h after the final administration of EPMP, and mice were treated to collect livers and blood. The levels of serum ALT, AST, ALB, ALP, TBIL, DBIL and TBA were measured by automatic biochemical analyzer, and liver tissues were taken for pathological examination. Results ANIT 80 mg·kg-1 significantly increased the activities of plasma ALT, AST, ALP, TBil, DBil, TBA, decreased the levels of ALB and injured liver compared with control group. Upon EPMP 125, 250 and 500 mg·kg-1, the transaminase activity was markedly decreased and the level of ALB was elevated. At the same time, serum bilirubin, TBA and ALP levels were significantly reduced. The HE staining results demonstrated that EPMP remarkably attenuated liver damage including degeneration, necrosis and infiltration of inflammatory cells. Conclusion The extracts from Pteris multifida Poir have significant protective effects on ANIT-induced liver injury and cholestatic jaundice.