Effects of Nuciferine on Apoptosis of Human Hepatocellular Carcinoma Cell Line HepG2 and Its Mechanism

Abstract

Abstract: Objective To investigate the effects of nuciferine on apoptosis of human hepatocellular carcinoma cell line HepG2 and its mechanism. Methods The proliferation of nuciferine on HepG2 cells was determined by the assay of cell counting kit-8(CCK-8). The apoptotic morphology of HepG2 cells with Hoechst 33258 staining was observed under fluorescent microscope. Cell cycle distribution and apoptotic rate were evaluated by flow cytomety(FCM). Western Blot analysis was used to detect the protein expression of apoptosis. Results Nuciferine could inhibit the proliferation of human hepatocellular carcinoma cell line HepG2 in a dose and time dependent manner. Hoechst 33258 staining showed that the experimental group of cells shrank, apoptotic bodies formed, blue fluorescence deepened and flow cytometry (FCM) indicated that HepG2 cells were arrested in G0/G1 phase and the apoptotic rate increased with the increase of the concentration of nuciferine. Western Blot result showed that nuciferine inhibited the expression of NF- КB and Bcl-2 proteins while promoted the expression of Bax protein in a concentration-dependent manner. Conclusion Nuciferine could inhibit the growth and proliferation of HepG2 cells in a dose and time-dependent manner and induce the apoptosis of HepG2 cells. Also, HepG2 cells were arrested in G0/G1 phase. Furthermore, up-regulation of Bax protein as well as downregulation of NF-КB and Bcl-2 protein expression may be one of the important apoptotic inducing mechanisms.

Key words: nuciferine, HepG2 cells, apoptosis, NF-КB protein, Bcl-2 protein, Bax protein

CLC Number:

R965

LI Na ,SONG Jin-chun. Effects of Nuciferine on Apoptosis of Human Hepatocellular Carcinoma Cell Line HepG2 and Its Mechanism[J]. Chinese Journal of Pharmacovigilance, 2017, 14(12): 715-719.

References

[1] 合理用药国际网络(INRUD)中国中心组临床安全用药组,中国药理学会药源性疾病学专业委员会,中国药学会医院药学专业委员会，等. 中国用药错误管理专家共识[J]. 药物不良反应杂志，2014，16(6):321-326.
[2] Tilyard M, Dovey S, Hall K. Avoiding and fixing medical errors in general practice: prevention strategies reported in the Linnaeus Collaboration's Primary Care International study of medical errors[J]. N Z Med J，2005，118(1208):1264.
[3] Gaur S, Sinha A, Srivastava B. Medication errors in medicine wards in a tertiary care teaching hospital of a hill state in India[J]. A J Pharm and Life Sci，2012，2(1):56-63.
[4] 张亚南，陈宁. 医院药房常见差错隐患的分析及应对措施[J]. 中国药物警戒，2013，10(3):181-183.
[5] 卫生部. 医疗机构药事管理规定[EB/OL] (2011-03-30)[2017-01-18]. http://www.moh.gov.cn/mohyzs/s3585/201103/51113.shtml.
[6] National Coordinating Council for Medication Error Reporting and Prevention. NCC MERP Index for Categorizing Medication Errors [EB/OL] (2001-02-20) [2017-01-18].http://www.nccmerp.org/sites/default/files/indexColor2001-06-12.pdf.
[7] Patel M, Desai M, Shah S, et al. A study of medication errors in a tertiary care hospital[J]. Perspect Clin Res，2016，7(4):168-173.
[8] Mathaiyan J, Jain T, Dubashi B, et al. Prescription, transcription and administration errors in out-patient day care unit of a regional cancer centre in South India[J]. Asian Pac J Cancer Prev，2016，17(5):2611-2617.
[9] 李晓玲，张青霞，王育琴，等. 我国医疗机构用药错误大数据的分析与启示[J]. 药物流行病学杂志，2017，26(1):40-45.
[10] 陈宁, 王婷, 马莉. 345例用药错误原因分析及对策[J]. 中国药物警戒，2015，12(2):106-109.
[11] Berdot S, Sabatier B, Gillaizeau F, et al. Evaluation of drug administration errors in a teaching hospital[J]. BMC Health Services Research，2012，12:60.
[12] Esqué Ruiz M T, Moretones Su ol M G, Rodríguez Miguélez J M, et al. Medication errors in a neonatal unit: One of the main adverse events[J]. An Pediatr (Barc)，2016，84(4):211-217.
[13] Parand A, Garfield S, Vincent C, et al. Carers' medication administration errors in the domiciliary setting: a systematic review[J]. PLoS One，2016，11(12):e0167204.
[14] Keers R N, Williams S D, Cooke J, et al. Causes of medication administration errors in hospitals: a systematic review of quantitative and qualitative evidence[J]. Drug Saf，2013，36:1045-1067.
[15] Sears K, O'Brien-Pallas L, Stevens B, et al. The relationship between nursing experience and education and the occurrence of reported pediatric medication administration errors[J]. J Pediatr Nurs，2016，31(4):283-290.
[16] Winsvold Prang L, Jelsness-J rgensen L P. Should I report? A qualitative study of barriers to incident reporting among nurses working in nursing homes[J]. Geriatr Nurs，2014，35(6):441-447.