Study on Toxicity of Different Extractions of Sophorae Tonkinensis

Abstract

Abstract: Objective To explore the major toxic constituent of Sophorae Tonkinensis. Methods The decoction of Sophorae Tonkinensis were prepared by the usage method of clinic, and then, the alkaloid part and non-alkaloid part (which mainly include flavonoids and saponins) of the decoction were prepared by macroporous resin and polyamide resin. The acute toxicity test of zebrafish was used to evaluate the toxicities of different extractions. In addition, MTT method was adopted to observe the impact of different extractions on growth of HepG2 cells, screening fractions with damage effect and caculating the half inhibitory concentration (IC50). LDH release assay was used to detect cell membrane injury. Flow cytometry analysis was used to detect apoptosis of HepG2 cells with Annexin V-FITC/PI double dye. Results The results of the toxicity of zebrafish showed that the non-alkaloid part was the main toxic part of Sophorae Tonkinensis. MTT assay also showed that the non-alkaloid site had the strongest inhibitory effect on the viability of HepG2 cells. The non-alkaloid part could induce HepG2 cell membrane injury and the rate of injury reached 67.31% at the concentration of 2 mg·mL^-1. According to flow cytometry analysis, the apoptosis of HepG2 cells induced by non-alkaloid part was higher than that of control (P <0.05). Conclusion All the above experiments suggest that the non-alkaloid part of Sophorae Tonkinensis may be the main toxic part. And the non-alkaloid part may affect cell viability by inducing cell membrane damage and apoptosis.

Key words: Sophorae Tonkinensis, liver cancer cell, zebrafish, toxicity, LDH, apoptosis

CLC Number:

R965

CHEN Ying,ZHANG Qian,HAN Shu-xian,HAN Fu-man,CHEN Liang-mian,TONG Yan,YOU Yun . Study on Toxicity of Different Extractions of Sophorae Tonkinensis[J]. Chinese Journal of Pharmacovigilance, 2017, 14(10): 582-586.

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