Influence of Alcohol Precipitating on Acute Toxicity and Toxicity Target Organs of Water Extracts from Herba Leonuri in Mice

Abstract

Abstract: Objective To study the influence of alcohol precipitating on water extracts from Herba Leonuri for acute toxicity, the damage degree of toxicity target organs in mice. Method UV spectrophotometry was used to measure the content of total alkaloids, the method of acute toxicity was used to compare the difference of acute toxicity between different water extracting processes. The toxic symptoms, cumulative mortality, weight changes of mice were recorded. Seven days after administration, blood was taken, the contents of serum biochemical indicators were detected while the liver, kidney were taken for organ coefficient. Results The contents of total alkaloids of Herba Leonuri in water extracts, alcohol precipitated water extracts were separately0.50%, 0.27%. MTD of water extracts, alcohol precipitated water extracts were88.0g·kg-1, 187.5g·kg-1, which were respectively equal to205.3, 437.5times of70kg human daily dried medicinal herb doses. There was one mouse died in each group. Compared with normal control group, the water extracts, alcohol precipitated water extracts from Herba Leonuri all could lead to significant increase of mice's BUN, Cr levels, kidney coefficient increased significantly. Compared with alcohol precipitated water extracts, the BUN, Cr levels of water extract group increased, kidney coefficient increased significantly. Compared with normal control group, the ALT, AST levels of water extract group increased. Alcohol precipitated water extracts could lead to AST level increasing, liver coefficient increased. Compare with alcohol precipitated water extracts, the ALT, AST, liver coefficient of water extract increased. Liver, kidney pathology showed that there were no obvious pathological changes in the liver, kidney tissues of mice. Conclusion Acute toxicity test showed that the toxicity target organs of Herba Leonuri were mainly kidney, liver. It proved that BUN, Cr levels significantly increased, ALT, AST levels increased, liver, kidney coefficients increased. The toxicity of kidney was higher than that of liver, the toxicity of water extract was higher than alcohol precipitated water extract. Combined with assay data of water extract, alcohol precipitated water extract from Herba Leonuri, it is suggested that the toxicity of Herba Leonuri's extracts, total alkaloids content showed a certain correlation. The toxicity, content of total alkaloids significantly decreased through alcohol precipitating process. Maybe alcohol precipitating process removed water soluble impurities, but the material base is still to be further studied

Key words: Herba Leonuri, alcohol precipitating, water extract, acute toxicity, target organ

CLC Number:

R992

SUN Xiao-qian, HUANG Na-na, YIN Li-shun, LI Xiao-yu, LV Li-li, ZHAO Hong, SUN Rong. Influence of Alcohol Precipitating on Acute Toxicity and Toxicity Target Organs of Water Extracts from Herba Leonuri in Mice [J]. Chinese Journal of Pharmacovigilance, 2015, 12(12): 717-721.

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