Abstract: Objective To observe the chronic toxicity damage and convalescence of the volatile oil components from Evodia Fructus in rats. Methods The volatile oil components with different doses from Evodia Fructus were separately orally given to rats, and the high, middle and low lose-group were separately 0.12, 0.06, 0.012 mL·kg^-1·d-1 calculated by crude herbs. Besides the general conditions were observed ,the related indexes were detected, such as the routine control of blood,hepatic function,renal function,the metabolism condition of lipids and the glycometabolism indexes. The rats were dissected and the weight of heart, liver, spleen, lung, kidney, were precisely weighed and calculated the ratio of organ to body. The histopathologic examination of hepatic tissue was carried out. The remaining rats were not administrated, and recovered for 20 days, the recovery conditions of rats were observed, and the above indexes were also detected. Results The volatile oil components from Evodia Fructus with different doses could induce the body weight decreasing in rats,the level of alanine aminotransferase(ALT), aspartate aminotransferase (AST), alkaline phosphatase(ALP)and total protein(TPC) serum obviously increased, albumin (ALB) and the ratio of ALB to GLB in serum obviously decreased, the liver weight and the ratio of liver to body increased, and the hepatic tissue damage was obvious in the histopathologic examination of hepatic tissue. The above-mentioned changes gradually aggravated with dose increasing, and there was no obvious difference compared with control group. The influence on the content of total bilirubin (TBI), blood urea nitrogen(BUN), glucose(GLU), total cholesterol(CHO) and creatinine(Cr) was not obvious, and the hepatic toxicity degree had certain dose dependence relatonship. Except the ALT level of different doses group in the above indexes of recovered rats had significant difference compared with control group, as well as the AST level of high dose group also had significant difference, the other indexes had no significant difference. Conclusion The long term administration of volatile oil components from Evodia Fructus at certain dose in rats could induce obvious cumulative toxicity, and the mainly toxical injury sites were in liver, but after recovery period observation, the live injury was basically recovered.

Key words: volatile oil components from Evodia Fructus, rat, chronic toxicity