Abstract: Objective To observe the long-term toxicity of the extracts of Phyllanthus urinaria L. in rats by repeated oral administration. MethodsDaily doses of extracts at 4.0 g·kg-1, 1.3 g·kg-1 and 0.5 g·kg-1 for 26 weeks were used for high, mid, and low dose group, respectively. Examinations including haematological indexes, biochemical indexes, and histopathology were performed at 13 weeks, 26 weeks during the administration, and 6 weeks after the withdrawal, respectively. ResultsThe rats in the group apperanced depressed and the fur lack luster during the administration. The levels of WBC, NE%, ALT, AST, and CR in high group were significantly higher than those in control group, and the levels of LY%, PLT, ALB in high group were significantly lower than those in control group at 26 weeks after administration(P <0.05 or P <0.01). Those changed indexes of hematology and blood biochemistery regained normal except NE% and LY% at 6 weeks after withdrawal. The liver of rats in high group and kidney of rats in high and mid group appeared reversible pathological changes. ConclusionRepeated oral administration of the extracts of Phyllanthus urinaria L. at high dose could lead to reversible damage to liver and kidney function and pathology.

Key words: Phyllanthus Urinaria L., rat, long-term toxicity