Abstract: Objective To compare the acute toxicity of different components in Herba Menthae on mice which is all components, peppermint oil, water extract, make an observation and evaluation of acute toxicity to Herba Menthae in order to provide a basis for safe and reasonable medication in clinical. MethodsRespectively prepare the all components, peppermint oil, water extract, and compare the acute toxicity of different components in Herba Menthae on mice in accordance with classical acute toxicity test methods, and the experimental datas are calculated LD50 with the Bliss method, with the continuous administration and observation for 14 days. ResultsThe LD50 of peppermint oil is 1.4895 ml·kg·d-1 and the 95%confidence interval is 1.3508-1.6407 mL·kg-1·d-1; the water extract in Herba Menthae are unable to make LD50, MTD results calculated in accordance with crude drug content were respectively 64.0g·kg-1·d-1,which is equal to 746.7 times of 70kg people's daily dried medicinal herb expenses, the MLD of all component is 24.36 g·kg-1·d-1, which is equal to 284.2 times of 70kg people's daily dried medicinal herb expenses. ConclusionThe acute toxicity of different components in Herba Menthae on mice is: peppermint oil> water extract> all components. By comparing the acute toxicity of different components in Herba Menthae on mice, it is beneficial to make an observation and evaluation of acute toxicity to Herba Menthae herbs, provide experimental basis for further toxicity-based parts and toxical substances and provide a basis for safe and reasonable medication in clinical.

Key words: Herba Menthae, different components, mice, acute toxicity, compare