Abstract: Objective To study the "dose-time-toxicity" relationship of hepatotoxicity caused by single dose water extracted and alcohol extracted components from Rhizoma Menispermi in mice. Methods Mice are grouped according to different time or dose points, the death condition and toxicity of mice were observed, the activity of ALT、AST in serum were detected, liver index was calculated. Results Compared with the control group, ALT, AST level in serum of water extracted and alcohol extracted components are peaked after 4 hours' administration, and restored near normality after 48 hours; the water extracted components of(46.05~61.4)g ·kg-1 and alcohol extracted components of(8.45~15.02)g ·kg-1 have obvious damage to the liver organization, and ALT, AST in serum increased significantly with the dose increasing. Conclusion The water extracted and alcohol extracted components gavages certain dose one time to mice could cause obvious acute hepatic injury, and there were certain time-toxicity and dose-toxicity relationships.

Key words: Rhizoma Menispermi, water and alcohol extracted components, mice, hepatotoxicity, "dose-time-toxicity" relationship