Abstract: Objective To investigate the "dose-time-toxicity" relationship of hepatotoxicity caused by multiple dose water extracted and alcohol extracted components from Rhizoma Menispermi to mice. Methods Mice are grouped according to different time or dose points, the death condition and toxicity of mice were observed, the activity of ALT、AST、ALP、ALB、TBI in serum were detected, liver index was calculated. Results Compared with the control group, the level of ALT, AST, ALP, TBI in serum of water extracted and alcohol extracted components increased, however the level of ALB reduced; and changes of the enzymatic activity in serum increased significantly with the dose increasing. Conclusion The continuous administration of different components from Rhizoma Menispermi to mice can induce obvious hepatotoxicity injury, and there were certain time-toxicity and dose-toxicity relationships.

Key words: Rhizoma Menispermi, water and alcohol extracted components, mice, hepatotoxicity, "dose-time-toxicity" relationship