Abstract: Objective To study the "dose-time-toxicity" relationship of hepatotoxicity caused by single dose water extracted and volatile oil components of Folium Artemisiae Argyi to mice. MethodsMice are grouped according to different time or dose points, to observe the death condition and toxicity of mice, detect ALT、 AST in serum, calculate liver, spleen, thymus index. ResultsCompared with the control group, ALT, AST level in serum of water extracted components are peaked after 2 hours' administration, and restored near normality after 72 hours. ALT level in serum of volatile oil components are peaked after 4 hours' administration, while AST level in serum of volatile oil components are peaked after 6 hours' administration, and they all restored near normality after 72 hours. The water extracted components of (8.0~1.33) g·kg-1 and volatile oil components of (0.34~0.13) mL·kg-1 have obvious damage to the liverorganization, and ALT, AST in serum increased significantly with the dose increasing. ConclusionThe water extracted and volatile oil components gavaged certain dose one time to mice could cause obvious acute hepa-injury, and there were certain time-toxicity and dose-toxicity relationships.

Key words: Folium Artemisiae Argyi, water extracted and volatile oil components, mice, hepatotoxicity, "dose-time-toxicity" relationship