Determination of Arbutin in Sedum Purpureum by HPLC

Abstract

Abstract: Objective To establish a HPLC method for the determination of arbutin in Sedum Purpureum. Methods Agilent XDB C18(4.6mm×150mm, 5μm) column was applied, using methanol-water(20 80) as mobile phase with the rate of 0.8mL·min-1. The column temperature was room temperature and the detective wavelength was 282 nm. Results A good linearity was obtained over the range of 5~160μg for arbutin(r=0.9998). The average recovery(n=6) was 101.67%(RSD=3.06%). Conclusion The method is practical, convenient, accurate, and can be used as the determination of arbutin in Sedum Purpureum.

Key words: Sedum purpureum, Arbutin, HPLC

CLC Number:

R927.2

ZHANG Hai-feng, SUN Jian, TENG Kun. Determination of Arbutin in Sedum Purpureum by HPLC[J]. Chinese Journal of Pharmacovigilance, 2011, 8(6): 321-322.

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Determination of Arbutin in Sedum Purpureum by HPLC

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