Effect of Leonurine on Hippocampal Neurons in the Early Stage of Diabetic Rat Model

doi:10.19803/j.1672-8629.2019.09.01

Abstract

Abstract: Objective To study the effect of Leonurine(SCM-198) on hippocampus neurons and expression of glutamate transporter in early stage of STZ induced type 2 diabetic rat model. Methods The type 2 diabetic rat model was established by intraperitoneal injection of streptozocin after five-week high fat diet. The rats were randomly divided into control group, DM model group and the SCM-198 intervention group. The neurobehavioral index of rats was assessed in all groups. The fasting blood glucose (FBG), total cholesterol (TC) , triglyceride (TG) , high density lipoprotein-cholesterol (HDL-C), low density lipoprotein- cholesterol (LDL-C) and fasting insulin (FINS) were tested. The histopathological changes of hippocampus neurons were examined by HE staining. The alteration of related transporter protein expression in hippocampus was determined by Western blotting. Results Compared with DM model group, rats in the SCM-198+DM group took a shorter time to reach the platform for the first time (P <0.01), stayed longer in the target quadrant(P <0.05) and platform, and traversed the platform more frequently. The levels of FBG and LDL-C in the SCM-198 intervention group were significantly decreased (P <0.05 and P <0.01 separately). And the levels of TC and TG were also increased (P > 0.05). The weight of animals in SCM-198 intervention group increased significantly (P < 0.05), and the weight of the brains did not differ much, so the brain index decreased (P < 0.05). The result of HE staining showed that there were obvious signs of recovery in the hippocampus CA1 area in the SCM 198 intervention group, which was characterized by increasing density of neurons and pyramidal cells with clear level. According to the result of Western blot, the expression level of GLP1 protein was increased in SCM-198 intervention group, while the vGLUT1 protein was decreased, both with a statistically significant difference (P < 0.05). And the RT-PCR showed the same result with the WB method. Conclusion In the early stages of diabetes development, the hippocampus neuron in brain tissue was injured, which led to changes in the expression of glutamate transporter. The intervention of Leonurine had the potential on reduction of nerve damage in hippocampal neurons via adjusting the expression of glutamate transporter.

Key words: diabetic encephalopathy, leonurine, neural behavior, hippocampal neurons, transporter, GLT1, vGLUT1

CLC Number:

R965

WANG Qi, CHI Xin, XUE Xin, LIANG Longkun, QIN Wei, ZHAO Yan, TIAN Jinghui, CHEN Qiang. Effect of Leonurine on Hippocampal Neurons in the Early Stage of Diabetic Rat Model[J]. Chinese Journal of Pharmacovigilance, 2019, 16(9): 513-522.

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