中国药物警戒 ›› 2014, Vol. 11 ›› Issue (9): 524-527.

• 质量与工艺研究 • 上一篇    下一篇

HPLC法双波长同时测定赤芍中4种成分的含量

柳梦婷1, 方婧1, 孙昊2, 吴宏伟1*, 付梅红1, 杨洪军1   

  1. 1中国中医科学院中药研究所,北京100700;
    2首都医科大学中医药学院,北京 100069
  • 收稿日期:2014-06-12 修回日期:2016-02-03 出版日期:2014-09-08 发布日期:2016-03-02
  • 通讯作者: 吴宏伟,博士,助理研究员,中药质量控制、药物分析研究。E-mail：whw9905012@163.com
  • 作者简介:柳梦婷,女,在读硕士研究生,中药学。
  • 基金资助:
    基金项目：国家重大新药创制专项: 中药大品种脑心通胶囊技术改造研究,课题编号：2011ZX09201-201-26

HPLC Ddouble Wavelength Simultaneous Determination of Four Components in Paeonia lactiflora Pall

LIU Meng-ting1, FANG Jing1, SUN Hao2, WU Hong-wei1, *, FU Mei-hong1YANG Hong-jun1   

  1. 1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
    2Capital Medical University School of Traditional Chinese Medicine, Beijing 100069, China
  • Received:2014-06-12 Revised:2016-02-03 Online:2014-09-08 Published:2016-03-02

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摘要： 目的采用高效液相色谱法,在不同检测波长下同时测定赤芍中儿茶素、芍药苷、苯甲酸、丹皮酚4种成分的含量。方法色谱柱为Diamonsil C18 5 m 250×4.6 mm,以0.1%甲酸-乙腈为流动相进行梯度洗脱,检测波长为230 nm（儿茶素、芍药苷、苯甲酸）,275 nm（丹皮酚）,流速1 mL·min-1,柱温30 ℃。结果不同产地赤芍中4种成分含量差异较大,7批不同产地赤芍药材中儿茶素、芍药苷、苯甲酸和丹皮酚的含量范围分别为：0%~0.052%、1.044%~4.326%、0.126%~0.249%、0.022%~1.060%。结论本法操作简便、准确、重复性好,可用于赤芍药材中儿茶素、芍药苷、苯甲酸、丹皮酚含量的测定。

关键词: 赤芍, 高效液相色谱法, 儿茶素, 芍药苷, 苯甲酸, 丹皮酚, 含量测定

Abstract: ObjectiveTo establish a HPLC method for determining the content of catechin, paeoniflorin, benzoic acid and paeonol in Paeonia lactiflora Pall at different wavelength. MethodsIt was performed on Diamonsil colunm C18 5 m 250×4.6 mm, mobile phase was 0.1% methanoic acid-methyl cyanide, gradient elute, detection wavelength was 230 nm (catechin, paeoniflorin, benzoic acid) and 275 nm (paeonol), flow rate was 1mL·min-1, and colunm temperature was 30℃. ResultsThe contents of four active components in Paeonia Lactiflora Pall from different habitats were quite different. The contents of catechin, paeoniflorin, benzoic acid and paeonol were 0%~0.052%, 1.044~4.326%, 0.126~0.249%, 0.022~1.060% respectively. ConclusionThe method is simple and accurate with good reproducibility, it is suitable for determining the contents of catechin, paeoniflorin, benzoic acid and paeonol in Paeonia lactiflora Pall.

Key words: Paeonia lactiflora Pall, HPLC, catechin, paeoniflorin, benzoic acid, paeonol, quality control

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