中国药物警戒 ›› 2010, Vol. 7 ›› Issue (11): 656-659.

• 半夏毒性影响因素研究 • 上一篇    下一篇

提取工艺对半夏急性毒性与相关物质基础含量的影响研究

黄伟12 张作平3 黄幼异2 鲍志烨2 孙蓉1   

  1. 1.山东省中医药研究院,山东济南250014；
    2.山东中医药大学,山东济南250355；
    3.济南杏林生物技术有限公司,山东济南250101
  • 收稿日期:2010-08-20 修回日期:2016-03-09 出版日期:2010-11-08 发布日期:2016-03-09
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理。 E-mail： sunrong107@163.com
  • 作者简介:黄伟,男,在读硕士研究生,中药药理与毒理。
  • 基金资助:
    国家重点基础研究发展计划（973）中医基础理论专项资半夏的毒性组分,并通过混淆品、产地、炮制等因素对半助项目（2009CB522802）；山东省科技平台建设项目（2008GG2NS0 夏水提组分毒性大小与相关物质基础含量影响研究,发2021）；山东省中医局科技发展计划课题（2007-119）

Experimental Researc h on the Influences of Extracting Process on the Content of Toxical Substances and Acute Toxicity of Rhizoma Pinelliae

HUANG Wei1,2 ,ZHANG Zuo-ping3, HUANG You-yi2, BAO Zhi-ye2 ,SUN Rong1   

  1. 1..Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2..Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;
    3..Jinan Xinglin Biology Techonology Corporation, Shandong Jinan 250101, China
  • Received:2010-08-20 Revised:2016-03-09 Online:2010-11-08 Published:2016-03-09

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摘要： 目的 考察提取工艺对半夏相关毒性物质基础含量与急性毒性的影响。方法 运用UV 法、HPLC 法、酸碱滴定法和经典急性毒性实验法,进行半夏不同提取工艺样品的含量测定和急性毒性比较研究。结果 半夏不同提取工艺样品总有机酸含量：75%醇渗液＞75%醇提液＞30%醇提液＞30%醇渗液＞水提组分抑水渗液；总生物碱含量：75%醇渗液＞75%醇提液＞30%醇渗液＞30%醇提液＞水渗液抑水提组分。毒性研究结果显示：75%醇渗液和75%醇提液可测出MTD 值,其余组测得MLD 值,毒性大小依次为75%醇渗液＞75%醇提液＞30%醇渗漉组＞30%醇提组＞水渗组＞水提组。结论 提取工艺对半夏毒性及相关毒性物质含量有一定的影响,其毒性大小与总生物碱、总有机酸含量呈一定相关性。

关键词: 半夏, 提取工艺, 毒性物质基础, 急性毒性

Abstract: Objective To compare the content of toxical substances and the acute toxicity of Rhizoma Pinelliae in different processing, and evaluate the processing's influences on the content of toxical substances and acute toxicity. Methods UV method, HPLC method, acid -base titration and the classical method of acute toxicity were used to determine the content of toxical substances and compare the acute toxicity of Rhizoma Pinelliae in different processing. Results The content of total organic acid is: 75% alcohol-infiltration>75% alcohol-extraction>30%alcohol-extraction> 30% alcohol -infiltration >water -infiltration抑water -extraction; the content of total alkaloid is: 75% alcohol -infiltration> 75% alcohol -extraction >30% alcohol -infiltration >30% alcohol -extraction >water -infiltration抑water -extraction. MTD value can betested in 75% alcohol -infiltration and 75% alcohol -extraction groups, while MLD value can be tested in other groups. The comparation of acute toxicity is: 75% alcohol-infiltration>75% alcohol-extraction>30% alcohol-infiltration>30% alcohol-extraction >water-infiltration >water -extraction. Conclusion Extraction has certain influence on the content of toxical substances and acute toxicity of , and the toxicity of Rhizoma Pinelliae has some relevance with the content of total organic acid and total alkaloid.

Key words: Rhizoma Pinelliae , extraction, toxical substances, acute toxicity

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