舒筋定痛片对斑马鱼胚胎发育的急性毒性研究

doi:10.19803/j.1672-8629.20210529

中国药物警戒 ›› 2023, Vol. 20 ›› Issue (3): 262-265.
DOI: 10.19803/j.1672-8629.20210529

舒筋定痛片对斑马鱼胚胎发育的急性毒性研究

刘静¹, 王峰¹, 张靖溥², 孟杰², 戴忠^1#, 马双成^1,*

¹ 中国食品药品检定研究院,北京 100050;
² 北京协和医学院,中国医学科学院医药生物技术研究所,北京 100050

收稿日期:2021-05-21 出版日期:2023-03-15 发布日期:2023-03-17
通讯作者: ^* 马双成,男,研究员·博导,中药民族药物质基础与质量安全评价。E-mail：masc@nifdc.org.cn;^#为共同通信作者。
作者简介:刘静,女,博士,中药分析、中药标准物质与中药质量控制。
基金资助:
重大新药创制国家科技重大专项2018年度（2018ZX09735-006）

Toxic effect of Shujin Dingtong tablets on embryonic development of zebrafish

LIU Jing¹, WANG Feng¹, ZHANG Jingpu², MENG Jie², DAI Zhong^1#, MA Shuangcheng^1,*

¹National Institutes for Food and Drug Control, Beijing 100050, China;
²Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China

Received:2021-05-21 Online:2023-03-15 Published:2023-03-17

摘要/Abstract

摘要： 目的采用斑马鱼胚胎模型评价舒筋定痛片样品的急性毒性。方法以模式生物斑马鱼胚胎为实验对象,以不同硼砂含量的舒筋定痛片样品给药处理,野生型为阴性对照,于不同发育时间点观察给药后胚胎的发育情况,包括胚胎致畸、致死检测。结果舒筋定痛片4组测试样品在不同浓度下对斑马鱼胚胎发育有明显影响,高浓度时胚胎毒性以导致胚胎发育停滞为主,中浓度时可致发育滞后并出现心脏、脑、躯干等主要器官畸形,低浓度时大多数胚胎发育接近正常,少数胚胎出现胚胎发育中轻度的滞后。结论舒筋定痛片测试样品对斑马鱼胚胎发育的影响主要表现为高、中浓度下的胚胎发育停滞、滞后;同时,研究表明同一企业样品毒性作用与其中所含硼砂存在一定剂量依赖性正相关。

关键词: 舒筋定痛片, 斑马鱼, 胚胎发育, 急性毒性, 硼砂, 停滞, 滞后

Abstract: Objective To study the toxic effect of Shujin Dingtong tablets on embryonic development of zebrafish. Methods Zebrafish embryos were treated with multiple groups of samples while the wild type was used as the negative control. Embryonic development was observed at different time points. Results The four groups of Shujin Dingtong tablets showed obvious effects on the embryonic development of zebrafish at different concentrations. At a high concentration, embryonic toxicity was mainly manifested in developmental stagnation while at a medium concentration, developmental retardation and malformation of the main organs including the heart, brain and trunk were observed. At a low concentration, most of the embryos were close to normal, and a small number of embryos showed mild developmental delay. Conclusion The main manifestations of the effect of Shujin Dingtong tablets on zebrafish are embryonic development stagnation and lag at high and medium concentrations. Additionally, the toxicity of samples from the same manufacturer has a dose-dependent positive relationship with the amount of borax contained.

Key words: Shujin Dingtong tablet, zebrafish, embryonic development, acute toxicity, borax, stagnation, lag

中图分类号:

刘静, 王峰, 张靖溥, 孟杰, 戴忠, 马双成. 舒筋定痛片对斑马鱼胚胎发育的急性毒性研究[J]. 中国药物警戒, 2023, 20(3): 262-265.

LIU Jing, WANG Feng, ZHANG Jingpu, MENG Jie, DAI Zhong, MA Shuangcheng. Toxic effect of Shujin Dingtong tablets on embryonic development of zebrafish[J]. Chinese Journal of Pharmacovigilance, 2023, 20(3): 262-265.

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舒筋定痛片对斑马鱼胚胎发育的急性毒性研究

Toxic effect of Shujin Dingtong tablets on embryonic development of zebrafish

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