中国药物警戒 ›› 2022, Vol. 19 ›› Issue (12): 1309-1314.
DOI: 10.19803/j.1672-8629.20210518

• 基础与临床研究 • 上一篇    下一篇

吴茱萸不同提取方式差异化学物质和毒性物质基础研究

姬海南1,2, 佟娜3,#, 刘畅4, 赵潺1,2, 周晓娟1,2, 隋峰5, 沈国林1,2,*   

  1. 1中国检验检疫科学研究院化学品安全研究所,北京 100123;
    2国家市场监督管理总局食品质量安全重点实验室,北京 100123;
    3国家市场监督管理总局食品审评中心,北京100022;
    4中国医学科学院北京协和医学院药用植物研究所,北京 100094;
    5中国中医科学院中药研究所,北京 100170
  • 收稿日期:2021-05-20 出版日期:2022-12-15 发布日期:2022-12-21
  • 通讯作者: *沈国林,男,博士,副研究员,保健食品安全。E-mail：shengl@aqsiqch.ac.cn; #为共同通信作者。
  • 作者简介:姬海南,男,助理研究员,保健食品安全。
  • 基金资助:
    国家市场监督管理总局技术保障专项（2022YJ28）; 国家重点研发计划（2017YFF0211201）; 国家市场监督管理总局化学品质量安全检测能力提升专项（15002000000019）

Variations of chemical substances and toxic substances of Evodia rutaecarpa using different extraction methods

JI Hainan1,2, TONG Na3,#, LIU Chang4, ZHAO Chan1,2, ZHOU Xiaojuan1,2, SUI Feng5, SHEN Guolin1,2,*   

  1. 1Institute of Chemicals Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China;
    2Key Laboratory of Food Quality and Safety for State Market Regulation, Beijing 100123, China;
    3Center for Food Evaluation, State Administration for Market Regulation, Beijing 100022, China;
    4Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China;
    5Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100170, China
  • Received:2021-05-20 Online:2022-12-15 Published:2022-12-21

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摘要： 目的 研究吴茱萸不同提取方式存在差异性的化学物质以及引起肝脏毒性可能的主要物质基础。方法 分别制备吴茱萸水提组分、30 %乙醇提组分、50 %乙醇提组分和80 %乙醇提组分,采用植物组学方法找出差异性化学物质,利用软件预测方法筛查主要差异性化学物质的毒性终点。结果 吴茱萸水提与不同浓度醇提相比较获得的差异性化学物质总共有50种,其中最主要的差异性化学物质是吴茱萸生物碱,毒性终点筛查的结果显示吴茱萸生物碱存在安全风险,并揭示了吴茱萸醇提取物的毒性大于水提取物的原因。结论 不同提取方式可以使吴茱萸存在差异性化学物质以及吴茱萸生物碱可能是其毒性物质基础。

关键词: 吴茱萸, 植物组学, 毒性物质基础, 软件预测

Abstract: Objective To study the chemical substances of Evodia rutaecarpa extracted using different methods and the possible main material basis of hepatotoxicity. Methods The water extract, 30% ethanol extract, 50% ethanol extract and 80% ethanol extract of Evodia rutaecarpa were prepared respectively. The differential chemical substances were identified using the method of phytomics, and the toxicity of the main differential chemical substances was screened via software prediction. Results The results showed that there were 50 types of different chemical substances in water extraction and ethanol extraction of Evodia rutaecarpa, among which the most important one was Evodia alkaloids. The results of toxicity endpoint screening showed that there were safety risks in Evodia rutaecarpa alkaloids. The toxicity of Evodia rutaecarpa alcohol extract was higher than that of water extract. Conclusion Different extraction methods can make a difference to chemical substances of Evodia rutaecarpa, and Evodia alkaloids may be the basis of its toxic substances .

Key words: Evodia rutaecarpa, phytomics, toxic substance basis, software prediction

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