中国药物警戒 ›› 2010, Vol. 7 ›› Issue (11): 653-655.

• 半夏毒性影响因素研究 • 上一篇    下一篇

山东半夏不同炮制品相关毒性物质基础含量与急毒实验比较研究

黄伟12 黄幼异2 戴衍鹏1 张丽美1 孙蓉1   

  1. 1.山东省中医药研究院,山东济南250014；
    2.山东中医药大学,山东济南250355
  • 收稿日期:2010-08-18 修回日期:2016-03-09 出版日期:2010-11-08 发布日期:2016-03-09
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:黄伟,男,在读硕士研究生,中药药理与毒理。
  • 基金资助:
    国家重点基础研究发展计划（973）中医基础理论专项资助项目（2009CB522802）；山东省科技平台建设项目（2008GG2NS02021）；山东省中医局科技发展计划课题（2007-119）

Comparative Stu dy on the Content of Toxical Substances and Acute Toxicity of Shandong Pinelliae and its Processed Products

HUANG Wei1,2, HUANG You-yi2, DAI Yan-peng1, ZHANG Li-mei1 ,SUN Rong 1   

  1. 1.Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2.Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2010-08-18 Revised:2016-03-09 Online:2010-11-08 Published:2016-03-09

PDF (PC)

256

摘要： 目的 进行山东半夏不同炮制品相关毒性物质基础含量与急性毒性的比较研究,考察炮制对相关毒性物质基础含量与毒性的影响。方法 运用UV 法、HPLC 法、酸碱滴定法和经典急性毒性实验法,进行半夏、清半夏水提组分总生物碱、总有机酸及其可测单一成分的含量测定和急性毒性比较研究。结果 相关物质基础含量测定结果显示：总有机酸、总生物碱和鸟苷含量大小为半夏＞清半夏；琥珀酸、麻黄碱含量为清半夏＞半夏。毒性研究结果显示：半夏、清半夏水提组分MLD 值为301.2 g/kg 和374.4 g/kg,分别相当于临床70kg 成人每公斤体重日用量的2 342.7 倍和2912.0 倍。结论 炮制对山东半夏相关毒性物质含量与毒性有一定的影响,半夏毒性大于其炮制品清半夏。

关键词: 山东半夏, 炮制, 毒性物质基础, 急性毒性

Abstract: Objective To compare the content of toxical substances and the acute toxicity of Shandong Pinelliae's different extractions, and discuss the extraction's influences on the content of toxical substances and acute toxicity. Methods UV method, HPLC method, acid -base titration and the classical method of acute toxicity were used to determine the content of toxical substances and compare the acute toxicity of Pinelliae and its extraction. Results The content of total organic acid,total alkaloid and vernine is: Pinelliae> extraction;the content of amber acid and ephedrine is: extraction>Pinelliae .The MLD of Pinelliaeis 301.2g/kg,and its extraction is 374.4g/kg, which is respectively equal to2 342.7 times, 2 912.0 times of 70kg people's daily dried medicinal herb expenses. Conclusion Extraction has certain influence on the content of toxical substances and acute toxicity of Pinelliae, and the toxicity of Pinelliaeis higher than its extraction.

Key words: Shandong Pinelliae, extraction, toxical substances, acute toxicity

中图分类号: