中国药物警戒 ›› 2026, Vol. 23 ›› Issue (10): 1098-1107.
DOI: 10.19803/j.1672-8629.20260291

• 蒙药药效物质基础与作用机制研究专栏（二） • 上一篇    下一篇

蒙药小茴香三味汤对小鼠心肌缺血再灌注损伤保护作用机制研究

白璐璐1,2, 田金墁, 陆景坤1, 张钟月1, 张笑茹1, 宋晓霞1, 唐昊1, 于凌泽1, 郭娜1, 杜雨露1, 王敏杰1,3*   

  1. 1内蒙古医科大学基础医学院,内蒙古 呼和浩特 010110;
    2内蒙古医科大学第二附属医院,内蒙古 呼和浩特 010010;
    3内蒙古医科大学中药（蒙药）质量研究与药效评价重点实验室,内蒙古 呼和浩特 010059
  • 收稿日期:2026-04-09 出版日期:2026-10-15 发布日期:2026-10-16
  • 通讯作者: *王敏杰,男,博士,教授,心脑血管药理学。E-mail: wangminjie@immu.edu.cn
  • 作者简介:白璐璐,女,硕士,心脑血管药理学。Δ为并列第一作者。
  • 基金资助:
    国家自然科学基金资助项目（82260813）;内蒙古自治区人力资源和社会保障厅2022年度草原英才工程青年创新创业—层次项目（DC2300001103）

Protective effects of Mongolian medicine Xiaohuixiang Sanwei Tang against myocardial ischemia-reperfusion injury in mice and the mechanisms

Bai Lulu1,2, Tian Jinman, Lu Jingkun1, Zhang Zhongyue1, Zhang Xiaoru1, Song Xiaoxia1, Tang Hao1, Yu Lingze1, Guo Na1, Du Yulu1, Wang Minjie1,3*   

  1. 1Basic Medical Sciences, Inner Mongolia Medical University, Hohhot Inner Mongolia 010110, China;
    2The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot Inner Mongolia 010010, China;
    3Key Laboratory of Quality Research and Efficacy Evaluation of Traditional Chinese Medicine (Mongolian medicine), Inner Mongolia Medical University, Hohhot Inner Mongolia 010059, China
  • Received:2026-04-09 Online:2026-10-15 Published:2026-10-16

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摘要： 目的 探讨蒙药小茴香三味汤（Xiaohuixiang Sanwei Tang,XHX）预处理对小鼠心肌缺血再灌注损伤（Myocardial Ischemia-Reperfusion Injury,MIRI）的保护作用。方法 将72只雄性C57BL/6J小鼠随机分为假手术（Sham）组,模型（Model）组,复方丹参滴丸（Compound Danshen Dripping Pills,CDDP,0.11 g·kg-1）阳性对照组,小茴香三味汤低（XHX-L,0.65 g·kg-1）、中（XHX-M,1.3 g·kg-1）、高剂量（XHX-H,2.6 g·kg-1）组,共6组,各组12只构建MIRI模型,各组小鼠缺血30 min,再灌注24 h后进行心功能指标检测,TTC染色检测MIRI 24 h后心脏梗死面积,苏木精-伊红（HE）染色检测MIRI 24 h后观察心肌组织结构和细胞形态,Masson染色检测MIRI 24 h后心肌纤维化情况,心脏取血,分离血清,检测各组小鼠MIRI氧化应激后指标;构建H9c2心肌细胞缺氧/复氧（H/R）模型,通过CCK-8实验,在细胞层面确证XHX的保护作用;基于网络药理学方法预测药物活性成分与潜在靶点,绘制PPI网络图,对共有靶点实施GO及KEGG通路富集分析;采集Sham组、Model组及XHX-H组的心肌组织样本进行转录组测序,挖掘并锁定潜在的关键作用靶点。结果 TTC结果显示,XHX减少了小鼠MIRI 24 h后梗死面积。ELISA及试剂盒检测证明XHX降低MIRI 24 h后肌酸激酶同工酶MB（Creatine Kinase-MB, CK-MB）、乳酸脱氢酶（Lactic Dehydrogenase, LDH）、丙二醛（Malondialdehyde, MDA）水平,增加超氧化物歧化酶（Superoxide Dismutase, SOD）水平。HE染色结果显示XHX可改善MIRI 24 h后心肌病理改变。Masson染色结果显示XHX可有效减轻心肌缺血再灌注小鼠的心肌纤维化程度。H9c2细胞实验证实,XHX-M组干预能够有效减轻H/R诱导的心肌细胞损伤,对维持细胞活力发挥显著保护作用。转录组测序结合网络药理学分析得出XHX可能通过趋化因子调节心肌缺血疾病的进程。结论 XHX预处理可显著改善小鼠MIRI,缩小MIRI后的梗死范围,减轻心肌纤维化水平;同时还可抑制心肌细胞凋亡,减轻炎性细胞浸润及对抗氧化应激损伤,多途径缓解了心肌组织的受损程度,保护心脏。

关键词: 蒙药, 小茴香三味汤, 心肌缺血再灌注, 心肌梗死, 纤维化, 炎症反应, 氧化应激, 小鼠

Abstract: Objective To investigate the protective effects of pretreatment with Mongolian medicine Xiaohuixiang Sanwei Tang (XHX) against myocardial ischemia-reperfusion injury (MIRI) in mice. Methods Seventy-two male C57BL/6J mice were evenly and randomly divided into six groups: a sham operation group (Sham), model group (Model), compound Danshen dripping Pill (CDDP, 0.11 g·kg-1) positive control group, and low-dose (XHX-L, 0.65 g·kg-1), medium-dose (XHX-M, 1.3 g·kg-1), and high-dose (XHX-H, 2.6 g·kg-1) XHX groups. An MIRI model was established by 30 minutes of ischemia followed by 24 hours of reperfusion. Cardiac function parameters were recorded, TTC staining was used to detect myocardial infarct size after 24 hours of MIRI, HE staining was performed to observe myocardial tissue structure and cell morphology, and Masson staining was conducted to evaluate myocardial fibrosis. Blood was taken from the heart before the serum was separated to detect the indexes of MIRI oxidative stress in each group. A hypoxia/reoxygenation (H/R) model of H9c2 cardiomyocytes was constructed, and the protective effects of XHX were confirmed at the cellular level by CCK-8 assay. Network pharmacology was used to predict the active components and potential targets of the drug, construct a protein-protein interaction (PPI) network, and perform GO functional annotation and KEGG pathway enrichment analysis on overlapping targets using databases. Myocardial tissue samples from the Sham, Model, and XHX-H groups were collected for transcriptome sequencing to identify and verify potential key targets. Results TTC staining showed that XHX reduced the infarct size in mice 24 hours after MIRI. ELISA and assay kit results suggested that XHX decreased the levels of creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), and malondialdehyde (MDA) while increasing superoxide dismutase (SOD) levels 24 hours after MIRI. HE staining revealed that XHX ameliorated myocardial pathological changes following MIRI. Masson staining indicated that XHX effectively reduced the degree of myocardial fibrosis in mice with MIRI. H9c2 cell experiments confirmed that interventions with the XHX-M group effectively alleviated H/R-induced cardiomyocyte injury and exerted a significant protective effect against cell viability. Transcriptome sequencing combined with network pharmacology analysis revealed that XHX could regulate the progression of myocardial ischemic disease through chemokines. Conclusion XHX pretreatment alleviates MIRI-induced damage, reduces myocardial infarct size and fibrosis, and protects the heart in mice following MIRI.

Key words: Mongolian Medicine, Xiaohuixiang Sanwei Tang, Myocardial Ischemia-Reperfusion, Myocardial Infarction, Fibrosis, Inflammatory Response, Oxidative Stress, Mice

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