中国药物警戒 ›› 2024, Vol. 21 ›› Issue (3): 257-262.
DOI: 10.19803/j.1672-8629.20230591

• 基于多模型、多靶点的中药抗感染药效及机制研究专栏（二） • 上一篇    下一篇

颅痛宁颗粒对眶下神经缩窄环术诱导三叉神经痛大鼠的治疗作用研究

李舒冉1, 郭姗姗1, 高双荣1, 包蕾1, 耿子涵1, 赵荣华1, 张敬升1, 庞博1, 张宇1, 王雅欣1, 徐英利1, 曹姗1, 韩冰2,#, 崔晓兰1Δ, 孙静1,*   

  1. 1中国中医科学院中药研究所,北京100029;
    2黑龙江省济仁药业有限公司,黑龙江 哈尔滨,150025
  • 收稿日期:2023-09-21 出版日期:2024-03-15 发布日期:2024-03-18
  • 通讯作者: *孙静,女,博士,副研究员·硕导,中药药理。E-mail: jingziaiwei@126.com;#为共同通信作者。
  • 作者简介:李舒冉,女,博士,助理研究员,中药药理与新药研发。
  • 基金资助:
    国家自然科学基金资助项目（82304877）; 中央级公益性科研院所基本科研业务费专项资金资助（ZZ15-YQ-035）; 中国中医科学院科技创新项目（CI2021B015）

Effect of Lutongning granulethe to trigeminal neuralgia induced by chronic constriction injury of the infraorbital nerve in rats

LI Shuran1, GUO Shanshan1, GAO Suangrong1, BAO Lei1, GENG Zihan1, ZHAO Ronghua1, ZHANG Jingsheng1, PANG Bo1, ZHANG Yu1, WANG Yaxin1, XU Yingli1, CAO Shan1, HAN Bing2,#, CUI Xiaolan1Δ, SUN Jing1,*   

  1. 1Institute of Chinese Materia Medica, Beijing 100029;
    2Heilongjiang Province Jiren Pharmaly CO., LTD., Haerbin, Heilongjiang 150025
  • Received:2023-09-21 Online:2024-03-15 Published:2024-03-18

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摘要： 目的 研究颅痛宁颗粒对三叉神经痛模型大鼠的治疗作用,为其临床应用提供参考资料。方法 采用眶下神经缩窄环术（ION-CCI）制备大鼠三叉神经痛模型,大鼠随机分为正常组、假手术组、模型组、卡马西平阳性药组、颅痛宁高剂量组（2.70 g生药·kg-1·d-1）、颅痛宁低剂量组（1.35 g生药·kg-1·d-1）。Von Frey毛刷测定大鼠触须垫机械痛阈值,生化法检测血液血流变和凝血功能,HE染色观察眶下神经病理变化,Western blot技术检测三叉神经内P38和 P-P38的蛋白水平。结果 颅痛宁颗粒能提高模型大鼠的痛阈值（P<0.05,P<0.01）降低模型大鼠血浆黏度（P<0.05,P<0.01）和全血还原黏度（P<0.05,P<0.01）,提高模型大鼠血流变能力;提高模型大鼠血液凝血酶原时间（prothrombintime,PT）、活化部分凝血活酶时间（activated partial thromboplatin time, APTT）和凝血酶时间（thrombin time,TT）水平（P<0.05,P<0.01）,降低模型大鼠纤维蛋白原（fibrinogen,FIB）水平（P<0.01）,改善凝血功能;改善模型大鼠眶下神经的病理改变;并降低模型大鼠三叉神经内 P-P38的表达量（P<0.01）。结论 颅痛宁颗粒具有改善三叉神经痛作用,可能与其活血化瘀功能有关。

关键词: 三叉神经痛, 眶下神经缩窄环术, 颅痛宁颗粒, 活血化瘀, 三叉神经痛模型, 大鼠, 卡马西平

Abstract: Objective To investigate the therapeutic effect of Lutongning granules to rat with Trigeminal neuralgia, and provide reference materials for the clinical applications. Methods Trigeminal neuralgia was made by chronic constriction injury of infraorbital nerve in rats. Rats were randomly divided into normal group, sham group, model group, carbamazepine group, Lutongning high dose group (2.70 g crude drug·kg-1·d-1) and Lutongning low dose group (1.35 g crude drug·kg-1·d-1). Von Frey Brush brush was used to detect the mechanical pain threshold of rat whisker pad, biochemical method was used to detect the coagulation and hemostream function, HE staining was used to observe the microcirculation change of the infraorbital nerve after perfusion, western blot was used to detect the p38 and p-p38 expression in the trigeminal nerve. Results Lutongning granulethe increased the pain threshold in model rats (P<0.05, P<0.01), reduced the plasma viscosity (P<0.05, P<0.01) and the whole blood reduction viscosity in model rats (P<0.05, P<0.01), improved the flow ability in model rats, increased PT, APTT and TT levels in model rats (P<0.05, P<0.01), reduced FIB levels (P<0.01), improved the microcirculation on the infraorbital nerve in the model rats, reduced the p-p38 expression in the trigeminal nerve of model rats (P<0.01). Conclusion Lutongning granules have the effect of improving trigeminal neuralgia, which may be related to the function of promoting blood circulation and removing blood stasis.

Key words: trigeminal neuralgia, chronic constriction injury of the infraorbital nerve, Lutongning granulethe, promoting blood circulation and removing blood stasis, trigeminal neuralgia, rat, carbamazepine

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