Abstract: Objective To study the "dose-time-toxicity" relationship of hepatotoxicity caused by Yangxue Shengfa capsules to mice. Methodse Study on the "time-toxicity" relationship: Ig Yangxue Shengfa capsules of certain dosage to mice and the changes of the level of ALT, AST, TBI in serum and organ index of liver, spleen and thoracic gland were tested at different time points after administration. Study on the "dosage-toxicity" relationship: Ig Yangxue Shengfa capsules of different dosages to mice and the corresponding treatment was performed at 2h after administration in accordance with the method of study on"time-toxicity" relationship. Results The study on the "time-toxicity" relationship indicated that the level of ALT,AST,TBI in serum reached to the peak at 2h after once administration of Yangxue Shengfa capsulesof higher dosage and all recovered to normal value at 8h after ig. The liver index raised to its topmost at 12h. and recovered to normal value at 48h after ig. The study on the "dosage-toxicity" relationship indicated that conspicuous hepartotoxical injury can be induced by Yangxue Shengfa capsules of (2.50~12.00)ml·kg-1 and the level of ALT, AST,TBI increased significantly with the increase of dosage. Conclusion once oral administration of Yangxue Shengfa capsuleswith large dosage may induce acute hepatotoxical injury in mice and show an obvious "dosage-time-toxicity" relationship and Its hepatic injury mechanism needs further research.

Key words: Yangxue Shengfa capsules, mice, hepatotoxicity, "dose-time-toxicity" relationship