Abstract: Objcetive To establish the method for the content determination of curcumin in Jianghuang Granules. Methods HPLC method was adopted. Chromatographic conditions were as follows: Shimpack VP-ODS(150 mm×4.6 mm, 5μm) column was used with mobile phase consisted of acetonitrile-4％ phosphoric acid(48∶52) at a flow rate of 1.0 mL·min-1 under a detection wavelength at 430nm. The column temperature was 30℃. Results The linear range of Curcumin was 2.104~105.2 μg·mL^-1(r=0.999 8) with an average recovery rate of 99.68％(RSD=1.34％, n=9). Conclusion The method is simple, reliable and accurate, and can be used for the quality control of Jianghuang Granules.

Key words: Jianghuang Granules, curcumin, HPLC, content determination