Abstract: Objective To observe the Melatonin(MLT) on Adriamycin induced neonatal rat cardiomyocytes in primary protective effect of dose-effect relationship. Methods Cultured neonatal rat cardiomyocytes with Adriamycin Preparation of cardiac cell injury model, experimental and normal control group(C group), Adriamycin Group(ADM in the culture medium Jiaru A lmg/L) and 30 minutes before joining the Adriamycin given MLT 50,100,200 μmol·L-1 group(M1, M2, M3 group). Observation of melatonin on cultured neonatal rat cardiomyocytes in each group malondialdehyde(MDA) and MTT metabolic rate. Results Myocardial cell injury and Adriamycin group, protection group, MDA release melatonin significantly reduced(P <0.01), and MTT metabolic rate was significantly higher(P <0.01), with a dose-dependent effect. Conclusion MLT on Adriamycin induced a protective effect of myocardial cell injury and a dose-dependent relationship.

Key words: melatonin, Adriamycin, cardiomyocyte apoptosis, myocardial protection