Abstract: Objective The hepatotoxicity induced was studied after given multiple administration of Saikosaponins to mice. Methods The rats were divided into different groups of high, medium and low dose. besides the normal conditions were observed, the heart、 liver、 spleen、 lung、 kidney weigh and volume ratio were calculated, the changes of liver morphology was observed after administration. Results The liver weight and volume ratio was increased, while the rat's weight were decreased. Pathological examination showed different degree of liver pathology tissue damage, and the damage of liver in rats show an obvious dosage relationship. Conclusion the damage of liver show a relationship with the dose of Bupleurum chinense. Saikosaponins is the toxicity site, and this show a organic disease of liver after given Saikosaponins 15 days, the damage and necrosis were the main pathological changes.

Key words: Saikosaponins, alcohol elution, rats, liver toxicity, pathology