Study on Neuroprotective Effects of Water Extract of Fomitopsis Pinicola on Dopaminergic Neurons in vitro

Abstract

Abstract: Objective To observe the neuroprotective effects of water extract of Fomitopsis Pinicola on 1-Methyl-4-phenylpyridine (MPP+) induced apoptosis of mesencephala dopaminergic cells in vitro. Methods Mesencephalic dopamin-ergic neurons were labeled by tyrosine hydroxylase(TH) staining to observe the survival of THir neurons and the average length of neurite growth. The activity of mitochondrial respiratory chain complex I was analyzed by descent rate of nicotinamide adenine dinucleotide (NADH). The relative apoptosis rate was analyzed by PI staining. Results The survival of TH immuno-reactive(THir) neurons and the average length of neurite growth increased obviously after MPP+ induction, the activity of mitochondrial respiratory chain complex I decreased remarkably, and the apoptosis rate increased significantly. Compared with the MPP+ control group, the survival of THir neurons and the average length of neurite growth were increased by water extract of Fomitopsis Pinicola at doses of 50 and 25 μg·mL-1 respectively. Water extract of Fomitopsis Pinicola treatment(50, 25 and 12.5 μg·mL-1)dramatically increased the activity of mitochondrial respiratory chain complex I compared with MPP+ control respectively. Compared with the MPP+ control, the relative rates of PI-positive cells were significantly decreased by water extract of Fomitopsis Pinicola at doses of 50 and 25 μg·mL-1 respectively. Conclusion Water extract of Fomitopsis Pinicola showed significant neuroprotective effect on mesencephala dopaminergic cells induced by MPP+.

Key words: Fomitopsis Pinicola, dopaminergic neuron, Parkinson, apoptosis, mitochondrial respiratory chain complex I

CLC Number:

R971

GUO Shanshan, Wolf Dieter Rausch. Study on Neuroprotective Effects of Water Extract of Fomitopsis Pinicola on Dopaminergic Neurons in vitro [J]. Chinese Journal of Pharmacovigilance, 2018, 15(10): 582-586.

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