Hepatotoxicity of Short Time Oral Administration of Aqueous Extract of Polygoni Multflori Radix in Rats

Abstract

Abstract: Objective To investigate the sensitive hepatotoxicity related blood serum biochemical indicators in rats after short-time continuous oral administration of aqueous extract of Polygoni Multflori Radix (AEPMR). Methods Male SD rats were orally administered with low and high dose (10、30 g?kg-1) of AEPMR once a day for 14 d. During the administration period, observed the rat general condition and weighed rats every day, and calculated food utilization rate weekly. Detected the levels of ALT, AST, ALP, TBIL, DBIL, and IBIL in rats after 1 d and 14 d administration. 14 d AEPMR administration later, rats were euthanized and anatomized for general observation. The liver of rats was weighed to calculate liver coefficient and then made to slices stained with hematoxylin and eosin (HE staining) for histopathological analysis. Results Rats appeared tired, stool soft, urine color deepened and fur not bright after AEPMR administration. There was no significant difference among groups in liver coefficient, weekly food utilization and ALT, AST, ALP levels in serum after 1 d and 14 d administration. Compared with control group, TBIL of low-dose group (P<0.05) and TBIL and DBIL of high-dose group (P<0.01) were significantly increased after 1 d administration. TBIL, DBIL and IBIL of high-dose group were significantly increased after 14 d administration (P<0.01). Liver pathological analysis showed that in rats administered with high dose of AEPMR for 14 d, parts of the liver had observed vacuolization of hepatocytes and Kupffer cell hypertrophic hyperplasia, and fibroblasts hypertrophic hyperplasia in portal area. Conclusion Certain dose of AEPMR could induce increase of blood serum bilirubin and liver injury of hepatocyte injury in rats after short-time oral administration.

Key words: aqueous extract of Polygoni Multflori Radix, liver injury, total bilirubin, indirect bilirubin, direct bilirubin

CLC Number:

R992

QUAN Zheng-yang, CHEN Jing, LI Deng-ke, FEI Zhan-yang, WANG Zi-jian, ZHOU Ming, LI Kai-ming, SUN Zhen-xiao. Hepatotoxicity of Short Time Oral Administration of Aqueous Extract of Polygoni Multflori Radix in Rats[J]. Chinese Journal of Pharmacovigilance, 2017, 14(6): 321-325.

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