Abstract: Objective To observe the effects of bicyclol on α-naphthalene isothiocyanate (ANIT) -induced liver injury in ICR mice. Methods ICR mice were given intragastric administration bicyclol 200 mg·kg^-1 for five days continuously, oncedaily. Two hours after the third administration, the mice were treated with ANIT 110 mg·kg^-1 to establish the liver damage model. All animals were killed on 2 h after the fifth bicyclol administration. The activities of ALT, AST, ALP, TBIL,DBIL and TBA in serum were measured by automatic chemistry analyzer. 10% liver homogenate were prepared to test the MDA and SOD. Results ANIT 110 mg·kg^-1 could induce significant liver damage, the mortality rate of mice increased and the levels of serum ALT, AST, ALP, ALB, TBIL, DBIL and TBA were significantly increased. 200 mg·kg^-1 bicyclol had no even aggravating effects on the elevated level of the blood biochemical indexes as well as MDA and SOD. Conclusion Bicyclol aggravates ANIT-induced liver injury in mice probably and this should catch our attentions.

Key words: α-naphthalene isothiocyanate, liver injury, bicyclol.