Abstract: Objective To compare the acute toxicity of different components in evodia fructus on mice which is all components, water extraction components, alcohol extraction components and volatile oil, make an observation and evaluation of acute toxicity to evodia fructus herbs in order to provide a basis for safe and reasonable medication in clinical. Methods Respectively prepare the all components, water extraction components, alcohol extraction components and volatile oil, and compare the acute toxicity of different components in evodia fructus on mice in accordance with classical acute toxicity test Methods, and the experimental datas are calculated LD₅₀ with the Bliss method,with the continuous administration and observation for 14 days. Results The acute toxicity of different components in evodia fructus on mice is: volatile oil>all components>alcohol extraction components>water extraction components. The LD₅₀ of volatile oil in evodia fructus is 2.70 mL·kg^-1·d^-1 and the 95% confidence interval is 2.58~2.84 mL·kg^-1·d^-1; the all volatile oil in evodia fructus are unable to make LD₅₀, MTD Results calculated in accordance with crude drug content were respectively 15.6g·kg^-1·d^-1、70.6 g·kg^-1·d^-1 and 80.0 g·kg^-1·d^-1,which is equal to 242.7 times、1 098.2 times and 1 244.4 times of 70kg people's daily dried medicinal herb expenses. Conclusion The evodia fructus components、water extraction components、alcohol extraction components and ctus herb has a certain toxicity, which is consistent with the pharmacopoeia and documentations. By comparing the acute toxicity of different components in evodia fructus on mice, it is beneficial to make an observation and evaluation of acute toxicity to evodia fructus herbs, provide experimental basis for further toxicity-based parts and toxical substances and provide a basis for safe and reasonable medication in clinical.

Key words: evodia fructus, different components, mice, acute toxicity