Chinese Journal of Pharmacovigilance ›› 2010, Vol. 7 ›› Issue (3): 135-138.

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The Effect of Fosfomycin on the Renal Toxicity Induced by Isepamicin in Rat Tissue Cage Infection Model Caused by Ps. Aeruginosa Biofilm

FAN Yan1, LIU Ru2, WANG Rui2,*   

  1. 1Center for Drug Reevaluation, SFDA, Beijing 100045;
    2Dept. Clinical Pharmacology Chinese PLA General Hospital, Beijing 100853, China
  • Received:2016-02-19 Revised:2016-02-19 Online:2010-03-08 Published:2016-02-19

Abstract: Objective To evaluate the effect of fosfomycin on the renal toxicity induced by isepamicin in rat tissue cage infection model caused by Ps. Aeruginosa biofilm. Methords The tissue cage method was used to establish the animal model of local Ps. Aeruginosa biofilm infection. Rats were randomly divided into 4 groups: control group, negative control group, positive control group(isepamicin 300 mg·kg-1) and fosfomycin and isepamicin group, 6 rats each group. Renal toxicity indexes such as serum creatinine(SCr), blood urea nitrogen(BUN), urinary production(UPD) and urine n-acetyl-β-d-glucosaminidase(NAG) were determined at 0, 2 and 4 weeks after abdominal cavity admi-nistration. At last observed nephridial tissue pathology through light microscope and transmission electron microscope(TEM). Results Tissue cage experiment in rat showed that the level of SCr, BUN and NAG of the combination group were raised up than control groups after administration for 4 weeks but no significant differences statistically(P>0.05), and those of the isepamicin group were raised up remarkably than other groups(P<0.05). At 4 weeks the nephridial tissue pathology Results which were observated through light microscope and TEM showed the level of renal damage induced by the group of fosfomycin and isepamicin were lower than that of the group(P<0.05). Conclusion The animal experiment showed that fosfomycin may relieve renal damage induced by isepamicin.

Key words: fosfomycin, isepamicin, bacterial biofilm, Pseudomonas aeruginosa(PAO1), renal toxicity

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