Effect of Pomegranate Peel Tannin on Migration and Invasion of Bladder Cancer T24 Cells

Abstract

Abstract: Objective To investigate the effect of pomegranate peel tannin on the migration and invasion of human bladder cancer T24 cells. Methods Human bladder cancer T24 cells were used as target cells, and the effects of different concentrations of tannin on migration of T24 cells were observed by cell scratch test and Transwell migration test. Transwell invasion assay was used to determine the effect of pomegranate peel tannin on the invasion ability of T24 cells. Results The results of cell scratch test showed that the wound healing ability of T24 cells decreased significantly after pomegranate peel tannin treatment. After 48 h, the wound healing rate of blank control group was 52.04 %, 12.5 μg·mL-1 pomegranate peel tannin group was 18.21 %, and the differences were statistically significant compared with the blank control group (P <0.001). Transwell migration assay indicated that the number of T24 cells passing through the membrane was significantly decreased after treated with pomegranate peel tannin for 24 h (P <0.001). The results of Transwell invasion test showed that the number of cells in the pomegranate peel tannin group passing through the artificial basement membrane decreased significantly. It can decrease the invasive activity (P <0.001). Conclusion Pomegranate peel tannin can inhibit the metastasis of bladder cancer cells.

Key words: pomegranate peel tannin, bladder cancer, migration, invasion

CLC Number:

R932

ZENG Maolin, ZHOU Benhong. Effect of Pomegranate Peel Tannin on Migration and Invasion of Bladder Cancer T24 Cells[J]. Chinese Journal of Pharmacovigilance, 2018, 15(7): 385-388.

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