Abstract: Objective To observe the effects of Xiaoyao pill (XYP) on ovarian oxidative damage and apoptosis in Glycosides of Tripterygium Wilfordii (GTW)-induced ovulation obstacles rats. Methods The rat model of ovulation caused by GTW was successfully established. One hundred and thirty-six rats were randomly divided into control group, model group, XYP high-dose group and XYP low-dose group, with 34 rats in each group. Serum and ovarian tissues levels of Tbil, Dbil and T-AOC were detected by colorimetry, and the levels of MDA were detected by penthiobarbital. Apoptosis of ovarian granulosa cells was observed by TUNEL. Results Compared with control group, serum and ovarian tissues MDA level increased(P <0.01), while Tbil, Dbil and T-AOC levels decreased in the model group (all P <0.01), the apoptosis of ovarian granulosa cells was significantly increased in model group (P <0.01). After treatment with XYP, the levels of serum and ovarian tissues Tbil and Dbil were increased (all P <0.05), while the levels of ovarian tissues MDA in those groups were lower than model group (all P <0.05). Moreover, the apoptosis of ovarian granulosa cells was significantly lower than those in model group (all P <0.01). XYP high-dose group shows the best effect. Conclusion XYP could improve follicular development and ovulation obstacles by regulating ovarian oxidative damage and apoptosis.

Key words: Xiaoyao pill, GTW-induced ovulation obstacles rats, oxidative damage, apoptosis, ovary