Abstract: Objective To investigate the clinical distribution and drug resistance characteristics of Klebsiella pneumonia in different hospitals, to analyze KPC gene, and to provide the basis for rational drug selection. Methods The pathogenic bacteria were isolated from clinical samples and identified by the two hospitals in 2015 respectively. The sample characteristics in clinical departments and type distribution were investigated. The results about drug resistance were verified with K-B method. All the Klebsiella pneumonia resistant to imipenem and meropenem were selected, of which the KPC genes were detected by PCR. Results The 5075 and 1694 strains bacteria were isolated from two hospitals respectively, including 524 strains Klebsiella pneumoniae and 286 strains respectively, which distributed mainly in ICU and respiratory department and from sputum, urine and blood samples. The drug resistance of Klebsiella pneumoniae in Tianjin Fourth Hospital were compound sulfamethoxazole, ampicillin, nitrofurantoin, ampicillin / sulbactam and piperacillin in order, while those in the other hospital were compound sulfamethoxazole, ampicillin, nitrofurantoin, ampicillin / sulbactam and cefazolin orderly. Some Klebsiella pneumoniae resistant to imipenem and meropenem could express the KPC gene. Conclusion The distribution characteristics and sample types of the two hospitals were similar, and the drug resistance spectrum was slightly different. These indicated that the respiratory tract infection in the respiration department and ICU should be paid attention to, and the rational drug was selected according to the drug resistance gene expression.

Key words: Klebsiella pneumonia, clinical distribution, sensitive rate, drug resistance gene