Abstract: ObjectiveTo study the combination effect of fosfomycin and aminoglycosides on the formation of biofilm of Pseudomonas aeruginosa(PAO1) in vitro. MethodsThe modified plate culture method was used to establish biofilm model in vitro with which the morphology of PAO1 biofilms were observed and compared by scanning electron microscopy(SEM) after treated with different dosages of fosfomycin and aminoglycosides. ResultsSEM of PAO1 biofilms showed that bacteria were both bacillus surrounded by accidented material and the bacteria were connected each other with mucoid substance. The restraining effects on the formation of biofilm of combination groups were more than that of non-combination groups. ConclusionThere were obvious differences between combination of fosfomycin and aminoglycosides groups and non-combination groups in the formation of PAO1 biofilm. Synergistic action of fosfomycin to aminoglycosides is verified.

Key words: fosfomycin, aminoglycosides, bacterial biofilm, Pseudomonas aeruginosa(PAO1)