Abstract: ObjectiveTo offer experimental basement for the study of correlation between toxicity and efficacy by researching the toxical and side effects accompanied with anti-inflammation of water and alcohol extract from Rhizoma Menispermi. MethodsThe model of ear swelling by compound recipe of croton oil and granuloma by agar were made and model mice were intragastricaly treated with water and alcohol extract from Rhizoma Menispermi of different doses for 3 and 7 days continually. The anti-inflammatory effect on acute and chronic inflammation was observed. The activities of serum ALT, AST and contents of Cr, BUN were detected while the ratio of liver and kidney to body was measured. ResultsBoth ear swelling by croton oil and granuloma by agar can be inhibited by multiple intragastric administration of water and alcohol extract from Rhizoma Menispermi, and the inhibition rate inhanced with the increase of dosage, showing a certain dependency on dosage. The inhibition rate of alcohol extract is higher than water extract. In mice serum samples of alcohol extract and water extract, the activities of ALT, AST and contents of Cr, BUN increased, and both the ratio of liver and kidney to body enlarged. These above index changed significantly with doses and time. The changes of alcohol extract about these indexes were heavier than water extract. ConclusionInflammation can be significantly inhibited by both alcohol extract and water extrat in the dosage range of 1.2 to 4.7 mg ·kg-1 and show an obvious "dosage-efficacy" relationship. The anti-inflammatory degree increase with the enlarge of dosage, and the effect of alcohol extract is stronger than that of water extract;toxical and side effects of different degree can be caused to liver after administration of the two extracts in the dosage range of 1.2 to 4.7 mg ·kg-1 for 3 and 7days; toxical and side effects of different degree also can be caused to kidney after administration of the two extracts of 4.7 mg ·kg-1 for 3 and 7days. All the changesd of these indexes showed obvious "dosage-time-toxicity" relationship and the toxical and side effects caused by alcohol extract is larger than that caused by water extract. Therefore,there is a certain correlation between toxicity and efficacy of Rhizoma Menispermi dependence on the dose and time. However, what the "toxic" mechanism is, Whether the mechanisms between toxicity and efficacy is related, the "toxic" is the strict "toxic" or "side effects" all need to be confirmed by further studies.

Key words: anti-inflammatory, Rhizoma Menispermi, toxical and side effects