Abstract: ObjectiveTo explore the best refined process of the total Bupleurum saikosaponin. MethodsThe content of Saikoside A was assayed by HPLC. ODS-C18 column(150×4.6mm, 5μm) was used. The mobile phase consisted of methanol-water(30:70). The flow rate was 1mL ·min-1. The detection wavelength was 210nm. To compare refined ability of different macroporous resins by assaying Saikoside A by inspecting absorption flow rate, elution solvent and its usage, elution flow rate. ResultsThe calibration curve was in good linearity within the range of 50~250μg ·mL-1(r=0.9991), the average recovery was 99.22%(RSD=1.07%). The best conditions were: D101 macroporous resin, the absorption flow rate was 1BV ·h-1, the elution solvent was 70% ethanol and its usage was 5BV, the elution flow rate was 2BV ·h-1. The content of Saikoside A in the refined artical was about 3%. ConclusionThe assaying method was simple and had good repetitiveness, could be used to assay Saikoside A in the total Bupleurum saikosaponin refined article. The refined process of the total Bupleurum saikosaponin by D101 macroporous resin was feasible. The macroporous resin was recycling, so it had good applied prospect in the refined process of total Bupleurum saikosaponin.

Key words: total Bupleurum saikosaponin, Saikoside A, macroporous resin, refined process