Abstract: Objective To study the "dose-time-toxicity" relationship of hepatotoxicity caused by single dose water extracted and alcohol extracted components of Polygonum multiflorum to mice. Methodse Mice are grouped according to different time or dose points, to observe the death condition and toxicity of mice, detect ALT、AST in serum, calculate liver, spleen, thymus index. Results Compared with the control group, ALT, AST level in serum of water extracted components are peaked after 4 hours' administration, and restored near normality after 24 hours; ALT, AST level in serum of alcohol extracted components are peaked after 2 hours' administration, and restored near normality after 48 hours; The water extracted components of (5.5~30.75)g·kg-1 and alcohol extracted components of (8.5~24.5)g·kg-1 have obvious damage to the liver organization, and ALT, AST in serum increased significantly with the dose increasing. Conclusion The water extracted and alcohol extracted components gavaged certain dose one time to mice could cause obvious acute hepa-injury, and there were certain time-toxicity and dose-toxicity relationships.

Key words: Polygonum multiflorum, water and alcohol extracted components, mice, hepatotoxicity, "dose-time-toxicity" relationship