Determination of the Contents of Anemonin in Clematis mandshurica Rupr by HPLC

Abstract

Abstract: Objective To establish a HPLC method for the determination of anemonin in Clematis mandshurica Rupr. Methods Agilent XDB C18(4.6 mm×250 mm, 5μm) column was applied,using. methanol-water(40：60) as mobile phase with the rate of 0.8mL·min-1. The column temperature was room temperature and the detective wavelength was 220 nm. Results A good linearity was obtained over the range of 5.45~109μg·mL-1 for anemonin (r=0.9993). The average recovery(n=6) was 102.41%(RSD=2.78%). Conclusion The method is simple, sensitive,good reproducibility and used as the determination of Anemonin in Clematis mandshurica Rupr.

Key words: Clematis mandshurica Rupr, HPLC, anemonin

CLC Number:

R927.2

ZHANG Hai-feng, SUN Jian, TENG Kun. Determination of the Contents of Anemonin in Clematis mandshurica Rupr by HPLC[J]. Chinese Journal of Pharmacovigilance, 2011, 8(10): 585-586.

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Determination of the Contents of Anemonin in Clematis mandshurica Rupr by HPLC

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