Synergistic Effect of Pudilan Xiaoyan Oral Liquid Combined with Amoxicillin against Streptococcus Pneumoniae Infection

doi:10.19803/j.1672-8629.2021.04.11

Abstract

Abstract: Objective To study the synergistic effect of amoxicillin combined with Pudilan Xiaoyan oral liquid against Streptococcus pneumoniae infection. Methods ICR mice were intranasally infected with Streptococcus pneumoniae. The protective effect of amoxicillin combined with Pudilan Xiaoyan oral liquid against Streptococcus pneumoniae infection in mice was investigated by using lung indexes, lung tissue pathological scores and expressions of inflammatory factors as evaluation indexes. Results Amoxicillin combined with Pudilan Xiaoyan oral liquid could significantly inhibit the pneumonia induced by Streptococcus pneumoniae in mice. The combination index of amoxicillin (0.37 g·kg^-1·d^-1) and Pudilan Xiaoyan oral liquid (5.5 mL/kg^-1·d^-1) was 0.12, which indicated strong synergistic effect on infected mice. The lung index inhibition rate was 61.22%, and the lung index of the combination group was significantly lower than that of the model control group and the single drug group (P<0.05, P<0.01). The combination index of amoxicillin (0.37 g/kg^-1·dd^-1) and Pudilan Xiaoyan oral liquid (2.75 mL/kg^-1·d^-1) was 0.57, which indicated the synergistic effect on infected mice. The combination of the two drugs could significantly inhibit the weight loss of infected mice, ameliorate the pathological damage to lung tissue (P<0.01), and significantly inhibit the increase of serum inflammatory factors IL-6 (P<0.01), KC (P<0.05), TNF-α (P<0.01) and MCP-1 (P<0.01) induced by bacterial infection. Conclusion Amoxicillin combined with Pudilan Xiaoyan oral liquid can have synergistic therapeutic effect against pneumonia in mice infected with Streptococcus pneumoniae, which indicates the clinical applicability of this approach.

Key words: Pudilan Xiaoyan oral liquid, amoxicillin, Streptococcus pneumoniae, synergistic effect

CLC Number:

R969.2

SHI Yujing, BAO Yanyan, GUO Shanshan, SUN Jing, ZHAO Ronghua, BAO Lei, GENG Zihan, YAO Rongmei, GAO Yingjie, CUI Xiaolan. Synergistic Effect of Pudilan Xiaoyan Oral Liquid Combined with Amoxicillin against Streptococcus Pneumoniae Infection[J]. Chinese Journal of Pharmacovigilance, 2021, 18(4): 346-351.

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