Protective Effect of Icariin on Oxidative Damage of HUVECs Induced by Hydrogen Peroxide

doi:10.19803/j.1672-8629.2019.10.02

Abstract

Abstract: Objective To observe the protective effect and its possible mechanism of icariin (ICA) on oxidative damage of human umbilical vein endothelial cells (HUVECs) induced by hydrogen peroxide. Methods An oxidative stress injury model of HUVECs induced by hydrogen peroxide in vitro was established, to which icariin of different concentrations of icariin（12.5 μg·mL^-1, 25 μg·mL^-1, 50 μg·mL^-1) was added in different groups were added. Cell viability was measured by MTT colorimetry, and superoxide dismutase (SOD) activity in cells and lactate dehydrogenase (LDH) released in the supernatant were measured using the biochemical method. Furthermore, MCP-1 and ICAM-1 were detected by real-time fluorescence quantitative PCR (RT-PCR). Results Icariin of different concentrations could significantly increase the cell viability and SOD activity of the damaged cells, and reduce the release of LDH in the supernatant compared with the model group. The difference was statistically significant (P <0.01). Further analysis of the mechanism showed that the expressions of ICAM-1 and MCP-1 mRNA were down-regulated (P <0.01). Conclusion Icariin can improve the oxidation index of damaged cells, reduce the expressions of atherosclerosis-related cytokines, and improve the progression of such vascular diseases such as atherosclerosis.

Key words: icariin, HUVECs, hydrogen peroxide, atherosclerosis, MCP-1, ICAM-1

CLC Number:

R965

ZHANG Li, HUANG Dehong, SUN Fujun, LI Guihai. Protective Effect of Icariin on Oxidative Damage of HUVECs Induced by Hydrogen Peroxide[J]. Chinese Journal of Pharmacovigilance, 2019, 16(10): 581-587.

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