中国药物警戒 ›› 2011, Vol. 8 ›› Issue (5): 275-277.

• 临床研究 • 上一篇    下一篇

益肾乌发口服液对小鼠急性肝毒性“量-时-毒”关系研究

黄幼异1,2, 孙蓉1, 孙虎1   

  1. 1. 山东省中医药研究院,山东 济南 250014;
    2. 山东中医药大学,山东 济南 250355
  • 收稿日期:2015-08-27 修回日期:2015-08-27 出版日期:2011-05-10 发布日期:2015-08-27
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:黄幼异,女,在读硕士研究生,药理学。
  • 基金资助:
    山东省科技平台建设项目（2008GG2NS02021）

Study on The "Dosage-Time-Toxicity" Relationship of Hepatotoxicity Induced by Yi Shen Wu Fa Oral Liquid in Mice

HUANG You-yi1,2, SUN Rong1, SUN Hu1   

  1. 1. Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2. Shandong University of TCM, Shandong Jinan 250355, China
  • Received:2015-08-27 Revised:2015-08-27 Online:2011-05-10 Published:2015-08-27

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摘要： 目的 研究益肾乌发口服液单次给药致小鼠急性肝毒性的“量-时-毒”关系。方法 “时–毒”关系研究：小鼠灌胃一定剂量的益肾乌发口服液,分别于给药后不同时间检测血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、总胆红素（TBI）水平及肝、脾、胸腺等脏器指数的变化。“量–毒”关系研究：给小鼠灌胃不同剂量的益肾乌发口服液,于给药后2h按“时-毒”研究方法对小鼠进行相应处理。结果 小鼠血清ALT、AST、TBI水平均在灌胃较高剂量的益肾乌发口服液后2h达到高峰,给药后12h肝脏指数升至最高,给药后72h可恢复至正常值;剂量在（4.46~25）mL·kg-1之间肝脏可产生明显病理损伤,且随着剂量增大,ALT、AST、TBI水平升高显著。结论 小鼠单次灌胃一定剂量的益肾乌发口服液可造成急性肝毒性损伤,并呈现明显的“量-时-毒”关系。

关键词: 益肾乌发口服液, 小鼠, 肝毒性, “, 量-时-毒”, 关系

Abstract: Objective Study the "dosage-time-toxicity" relationship of hepatotoxicity induced by Yi Shen Wu Fa oral liquid in mice. Methods Study on the "time-toxicity"relationship: Ig Yi Shen Wu Fa oral liquid of certain dosage to mice and the changes of the level of ALT, AST, TBI in serum and organ index of liver, spleen and thoracic gland were tested at different time points after administration. Study on the "dosage-toxicity"relationship: Ig Yi Shen Wu Fa oral liquid of different dosages to mice and the corresponding treatment was performed at 2h after administration in accordance with the method of study on"time-toxicity"relationship. Results The study on the "time-toxicity" relationship indicated that the level of ALT, AST, TBI in serum reached to the peak at 2h after once administration of Yi Shen Wu Fa oral liquid of higher dosage and the liver index rised to its topmost at 12h. They all recovered to normal value at 72h after ig. The study on the "dosage-toxicity"relationship indicated that conspicuous hepartotoxical injury can be induced by Yi Shen Wu Fa oral liquid of (4.46~25)mL·kg-1 and the level of ALT, AST, TBI increased significantly with the increase of dosage. Conclusion Once oral administration of Yi ShenWu Fa oral liquid with large dosage may induce acute hepatotoxical injury in mice and show an obvious "dosage-time-toxicity" relationship.

Key words: Yi Shen Wu Fa oral liquid, mice, hepatotoxicity, "dosage-time-toxicity" relationship

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