中国药物警戒 ›› 2011, Vol. 8 ›› Issue (5): 278-280.

• 临床研究 • 上一篇    下一篇

益肾乌发口服液小鼠肝毒性“量-时-毒”关系的病理学研究

谢元璋1,2, 钱晓路3, 孙蓉1   

  1. 1. 山东省中医药研究院,山东 济南 250014;
    2. 哈尔滨商业大学,黑龙江 哈尔滨 150000;
    3. 山东中医药大学,山东 济南 250355
  • 收稿日期:2015-08-27 修回日期:2015-08-27 出版日期:2011-05-10 发布日期:2015-08-27
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:谢元璋,男,在读硕士研究生,药理学。
  • 基金资助:
    国家重点基础研究发展计划（973）中医基础理论专项资助项目（2009CB522802）;山东省科技平台建设项目课题（2008GG2NS02021）

Pathologic Research on Hepatotoxicity in Mice Caused by Yi Shen Wu Fa Oral Liquid

XIE Yuan-zhang1,2, QIAN Xiao-lu3, SUN Rong1   

  1. 1. Shandong Research Academy of TCM,Shandong Jinan 250014, China;
    2. Harbin University of Commerce, Heilongjiang Harbin 150000, China;
    3. Shandong University of TCM, Shandong Jinan 250355, China
  • Received:2015-08-27 Revised:2015-08-27 Online:2011-05-10 Published:2015-08-27

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摘要： 目的 研究益肾乌发口服液单次给药对小鼠造成的肝毒性在病理学上的损伤程度,为益肾乌发口服液肝毒性损伤提供病理组织学依据。方法 采用常规HE染色方法,通过光镜下阅片观察和病理图像分析,观察益肾乌发口服液单次灌胃对小鼠肝毒性“量-时-毒”关系研究中的病理学变化。结果 时-毒关系研究：给药后2h检测发现,在给药后6~24h表现较明显,量-毒关系研究：在10.56~25 mL·kg-1之间出现不同程度的病理组织学损伤,本品导致的主要病理改变为：肝细胞嗜酸性变、玻璃样变、脂肪变、空泡变等。结论 单次给小鼠灌胃不同剂量的益肾乌发口服液可造成急性肝损伤,并呈现一定的“量-时-毒”关系,并可导致肝细胞出现嗜酸性变、玻璃样变、脂肪变、空泡变等病理学改变。

关键词: 益肾乌发口服液, 肝毒性, 病理学, “, 量-时-毒”, 关系

Abstract: Objective To investigate the pathologic injury degree of hepatotoxicity in mice induced by single administration of Yi Shen Wu Fa oral liquid and provide pathohistological basis for its hepatotoxical injury. Methods The pathologic changes of mice in the study on "dose-time-toxicity"relationship of hepatotoxicity caused by Yi Shen Wu Fa oral liquid was observed with light microscope and image analysis after HE stain. Results The study on the "time-toxicity" relationship indicated that the hepatotoxicity occurred at 2h after administration and expressed obviously in the range from 6h to 24h after administration. The study on the "dose-toxicity"relationship showed that pathohistological injury of different degree can be induced by drug of (10.56~25.00) g·kg-1 and acidophilia, hyalinization, vacuolus were the main pathologic changes. Conclusion Once oral administration of Yi Shen Wu Fa oral liquid with certain dosage may induce acute hepatotoxical injury in mice and show an obvious "dose-time-toxicity"relationship. It can cause pathologic changes such as acidophilia, hyalinization, vacuolus to hepatic cells.

Key words: Yi Shen Wu Fa oral liquid, hepatotoxicity, pathology, "dose-time-toxicity" relationship

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