中国药物警戒 ›› 2012, Vol. 9 ›› Issue (5): 279-281.

• 管理及工作研究 • 上一篇    下一篇

提取方式对吴茱萸“质量-毒性”综合评价模式的影响

王会1，2，朱兰兰3，黄伟2，孙蓉2，*   

  1. 1 山东中医药大学，山东 济南 250355；
    2 山东省中医药研究院，山东 济南 250014；
    3 天津中医药大学，天津 300193
  • 收稿日期:2012-03-29 出版日期:2012-05-10 发布日期:2015-08-10
  • 通讯作者: 蓉,女,博士生导师,研究员,中药药理、毒理与新药研究。E-mail：sunrong107@163.com
  • 作者简介:王会，女，硕士研究生，中药药理与毒性研究。
  • 基金资助:
    国家重点基础研究发展计划 （973）中医基础理论专项资助项目 （2009CB522802）

The Effect of Extracting Methods on "Quality-Toxicity" Comprehensive Evaluation Model of Evodia Rutaecarpa(Juss.) Benth

WANG Hui1, 2, ZHU Lan-lan3, HUANG Wei2, SUN Rong2, *   

  1. 1 Shandong University of Traditional Chinese Medicine, Shandong jinan 250014, China;
    2 Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China;
    3 Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
  • Received:2012-03-29 Online:2012-05-10 Published:2015-08-10

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摘要： 目的对不同提取方式提取的吴茱萸中生物碱含量和急性毒性大小进行比较研究，探讨不同提取方式对吴茱萸“含量-毒性”综合评价模式的影响。方法用HPLC法测定吴茱萸水提组分、醇提组分中吴茱萸碱和吴茱萸次碱的含量，并进行方法学考察;经典急性毒性实验法，进行吴茱萸不同提取物急性毒性比较研究。结果吴茱萸水提物、醇提物中吴茱萸碱和吴茱萸次碱的百分含量分别为0.028%和0.027%、0.046%和0.075%，水提物组和醇提组无法作出LD50，MTD 试验结果按含生药量计算分别为80.0g·kg-1·d-1和70.6 g·kg-1·d-1。结论吴茱萸经不同方式提取后吴茱萸碱和吴茱萸次碱含量和毒性各不相同且各样品均出现死亡，其最大耐受量显示吴茱萸经不同方式的提取均具有毒性，其提取物对小鼠急性毒性强度为：醇提物组> 水提物组;提取物中吴茱萸碱和吴茱萸次碱的含量依次为：醇提物组> 水提物组。说明提取方式对吴茱萸“质量-毒性”综合评价模式有一定的影响，其毒性大小可能与主要化学成分含量存在一定相关性。因此通过“质量-毒性”综合评价模式确定有毒中药的提取方式，更全面、客观、合理。

关键词: 吴茱萸, 提取方式, 质量, 毒性, 综合评价

Abstract: Objective Explore the effect of extracting methods on "quality-toxicity" comprehensive evaluation model by comparing the content of alkaloid and acute toxicity from Evodia Rutaecarpa(Juss.) Benth extracted by different methods. MethodsHPLC was used to determine the content of Evodiamine and Rutaecarpine in water extract and alcohol extract of Evodia Rutaecarpa(Juss.) Benth; the classical method of acute toxicity was used to determine the acute toxicity of Evodia rutaecarpa(Juss.) Benth. at different extraction, and compare the acute toxicity. ResultsThe content of Evodiamine and Rutaecarpine which included water extract and ethanol extract was separately 0.028% and 0.027%,0.046% and 0.075%. The two groups are unable to make LD50, MTD results calculated in accordance with crude drug content were respectively 80.0 g·kg-1·d-1 and 70.6g·kg-1·d-1. ConclusionThe content of Evodiamine and Rutaecarpine and acute toxicity of the Fructus Evodiae Rutaecarpae by different extracting methods were different, and death occurred in each sample, the MLD showed that the different extracts which extracted from the Fructus Evodiae Rutaecarpae at different solvents extraction had certainly toxicity in mice. The acute toxicity of different extracts on mice is: alcohol extraction group>water extraction group, the content of Evodiamine and Rutaecarpine in different extracts is: alcohol extraction group>water extraction group. Suggesting that it was certain effect of extracting methods on "quality toxicity" comprehensive evaluation model. It was more comprehensive, Objective and reasonable to determine extraction methods of toxic Chinese medicine through the comprehensive evaluation model.

Key words: Evodia Rutaecarpa(Juss) Benth., evaluation model, quality, toxicity, comprehensive evaluation