中国药物警戒 ›› 2026, Vol. 23 ›› Issue (7): 739-745.
DOI: 10.19803/j.1672-8629.20260510

• 脓毒症中医药机制与临床用药研究专栏 • 上一篇    下一篇

苏合香丸对脓毒症心肌损伤小鼠的保护作用及剂量效应研究

杨卓雅1,2,3, 白颖璐1,2, 杨玉媚1, 耿宇立4, 徐霄龙1,2#, 刘清泉1,2,3,*   

  1. 1首都医科大学附属北京中医医院急诊危重症诊疗中心,北京 100010;
    2北京市中医药研究所,北京 100010;
    3辽宁中医药大学第二临床学院,辽宁 沈阳 110847;
    4清华大学玉泉医院内分泌科,北京 100084
  • 收稿日期:2026-06-22 出版日期:2026-07-15 发布日期:2026-07-16
  • 通讯作者: #为共同通信作者。*刘清泉,男,主任医师,教授•博导,中西医结合急诊与危重症。E-mail: liuqingquan2003@126.com
  • 作者简介:杨卓雅,女,在读硕士,中医内科学。
  • 基金资助:
    国家科技重大专项重大新药创制（2025ZD01903000）; 国家中医药管理局高水平中医药重点学科建设项目（ZYYZDXK-2023001）

Protective effects and dose effects of Suhexiang pills against septic myocardial injury in mice

Yang Zhuoya1,2,3, Bai Yinglu1,2, Yang Yumei1, Geng Yuli4, Xu Xiaolong1,2#, Liu Qingquan1,2,3,*   

  1. 1Emergency and Critical Care Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China;
    2Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China;
    3The Second Clinical College of Liaoning University of Traditional Chinese Medicine, Shenyang Liaoning 110847, China;
    4Department of Endocrinology, Yuquan Hospital, Tsinghua University, Beijing 100084, China
  • Received:2026-06-22 Online:2026-07-15 Published:2026-07-16

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摘要： 目的 探究苏合香丸对盲肠结扎穿孔（CLP）诱导脓毒症心肌损伤小鼠的保护作用及剂量效应关系,为脓毒症心肌病的中医药干预提供实验参考。方法 选取 SPF 级雄性C57BL/6J小鼠,随机分为假手术组,模型组,苏合香丸低剂量组和高剂量组（0.195、0.390 g·kg-1）。采用CLP法建立脓毒症心肌损伤模型,于回盲部连接杆下方结扎并固定,21G针头贯通穿刺1次,造模后连续灌胃给药7 d。观察小鼠一般状况与7 d生存率,检测超声心动图心功能参数,苏木精-伊红（HE）染色观察心肌与肺组织病理形态。结果 与假手术组相比,模型组小鼠精神萎靡、体重增长缓慢,7 d死亡率达70%;乳酸水平显著升高;左室射血分数、短轴缩短率显著下降,心室收缩末期容积、左心室收缩期内径升高;心肌纤维排列紊乱、溶解,肺组织炎细胞浸润明显。与模型组相比,苏合香丸2个剂量组均可改善小鼠一般状态,纠正酸碱失衡、降低乳酸含量,显著提高生存率、改善心功能指标并减轻组织病理损伤;其中高剂量在生存率提升、降低乳酸水平及组织损伤改善方面优势显著。结论 苏合香丸对CLP诱导的脓毒症心肌损伤小鼠具有保护作用,可改善全身内环境、提升心功能、减轻组织损伤并降低死亡率,药效存在剂量依赖性,低剂量和高剂量均可发挥保护效应,高剂量综合干预效果更佳。

关键词: 苏合香丸, 脓毒症心肌损伤, 盲肠结扎穿孔模型, 心功能, 剂量效应, 小鼠

Abstract: Objective To explore the protective effectsof Suhexiang pillsagainst septic myocardial injury induced by cecal ligation and puncture (CLP) in miceand dose-effect relationships in order to to provide data for interventionswith septic cardiomyopathyusing traditional Chinese medicine. Methods SPF male C57BL/6J mice were randomly divided into a sham operation group, model group, Suhexiang pill low dose group and high dose group (0.195, 0.39gdian·kg-1). A sepsis myocardial injury model was established using the CLP method. Ligation and fixation were performed under the connecting rod of the ileocecal junction, and a penetrating puncture was made with the 21 G needle. After modeling, the rats were intragastrically administrated for 7 consecutive days. The condition and 7-day survival rate of these mice were observed, the cardiac function parameters of echocardiography were detected, and the pathomorphology of myocardia and lung tissues was observed via HE staining. Results Compared with the sham operation group, the mice in the model group were depressed, weight gain was slow, and the 7-day mortality was 70%. The lactic acid level increased significantly, the left ventricular ejection fraction and short axis shortening rate decreased significantly while the ventricular end systolic volume and left ventricular systolic diameter increased. The myocardial fibers became disordered and dissolved, and the infiltration of inflammatory cells in lung tissue was pronounced. Compared with the model group, the general stateof the two dose groups of Suhexiang pills was improved, the acid-base imbalancecorrected, lactic acid contents reduced, survival rateincreasedsignificantly, cardiac function indexes improved and histopathological damage mitigated. A high dose had significant advantages in improving survival rate, reducing lactic acid levels and lessening tissue damage. Conclusions uhexiang pills have a marked protective effect against CLP-induced septic myocardial injury in mice by improving the internal environment of the whole body, enhancing cardiac function, reducing tissue damage and lowering mortality. The efficacy is dose-dependent. High-dose interventionsare more effective.

Key words: Suhexiang Pills, Sepsis Myocardial Injury, Cecal Ligation and Perforation Model, Cardiac Function, Dose Effect, Mice

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