中国药物警戒 ›› 2026, Vol. 23 ›› Issue (1): 26-33.
DOI: 10.19803/j.1672-8629.20250355

• 蒙药药效物质基础与作用机制研究专栏 • 上一篇    下一篇

蒙药萨乌日勒、珍宝丸、赞丹-3对小鼠脑缺血再灌注损伤保护作用的研究

宋晓霞1, 杨艳2, 周树宏1,3, 赵向东4, 张钟月1, 张笑茹1, 高倩1, 王敏杰1,3,*   

  1. 1内蒙古医科大学基础医学院,内蒙古 呼和浩特 010000;
    2内蒙古医科大学药学院,内蒙古 呼和浩特 010000;
    3内蒙古医科大学中药（蒙药）质量研究与药效评价重点实验室,内蒙古 呼和浩特 010000;
    4内蒙古医科大学附属医院,内蒙古 呼和浩特 010059
  • 收稿日期:2025-06-03 出版日期:2026-01-15 发布日期:2026-01-15
  • 通讯作者: *王敏杰,男,博士,教授·硕导,心脑血管基础药理研究。E-mail：wangminjie@immu.edu.cn
  • 作者简介:宋晓霞,女,硕士,心脑血管基础药理研究。
  • 基金资助:
    国家自然科学基金资助项目（81703511）; 内蒙古自治区高等学校创新团队发展计划（NMGIRT2311）; 内蒙古医科大学实验室开放基金指南性项目（2024ZN12）

Protective Effects of Mongolian Medicines Saurile, Zhenbao Pills and Zandan-3 against Cerebral Ischemia-Reperfusion Injury in Mice: a Comparative Study

SONG Xiaoxia1, YANG Yan2, ZHOU Shuhong1,3, ZHAO Xiangdong4, ZHANG Zhongyue1, ZHANG Xiaoru1, GAO Qian1, WANG Minjie1,3,*   

  1. 1School of Basic Medicine, Inner Mongolia Medical University, Hohhot Inner Mongolia 010000, China;
    2School of Pharmacy, Inner Mongolia Medical University, Hohhot Inner Mongolia 010000, China;
    3Key Laboratory of Quality Research and Efficacy Evaluation of Traditional Chinese Medicine (Mongolian Medicine), Inner Mongolia Medical University, Hohhot Inner Mongolia 010000, China;
    4The Affiliated Hospital of Inner Mongolia Medical University, Hohhot Inner Mongolia 010059, China
  • Received:2025-06-03 Online:2026-01-15 Published:2026-01-15

PDF (PC)

19

摘要： 目的 对比3种经典蒙药萨乌日勒、珍宝丸、赞丹-3对小鼠脑缺血再灌注损伤的保护效果,并选择最优方剂以进行深入机制研究。方法 120只C57BL/6J小鼠随机分为假手术组、模型组、尼莫地平组（46.8 mg·kg-1）、萨乌日勒组（338 mg·kg-1）、珍宝丸组（780 mg·kg-1）和赞丹-3组（1 950 mg·kg-1）,每组20只。给药组分别灌胃10 d,假手术组及模型组均给予同体积生理盐水,然后采用线栓法建立短暂性大脑中动脉闭塞模型,缺血1.5 h再灌注24 h,假手术组只进行手术不插入线栓。再灌注24 h后取脑组织分别进行TTC染色评价缺血面积,进行苏木精-伊红（HE）染色和尼氏染色观察病理改变,观察小鼠行为学进行评分;取血清分别检测超氧化物歧化酶（Superoxide Dismutase, SOD）、丙二醛（Malondialdehyde, MDA）、乳酸脱氢酶（Lactate Dehydrogenase, LDH）水平,对比蒙药萨乌日勒、珍宝丸、赞丹-3对脑缺血再灌注损伤的保护作用,通过网络药理学对蒙药萨乌日勒进行靶点预测。结果 与假手术组比,模型组TTC染色梗死面积显著增高,神经行为学评分显著降低（P<0.001）,HE染色明显可见梗死病灶,尼氏染色可观察到神经元损伤明显（P<0.001）,血清SOD、MDA、LDH活力明显降低（P<0.001）。与模型组相比,尼莫地平组、萨乌日勒组、珍宝丸组、赞丹-3组TTC染色结果显示梗死区域明显减轻（P<0.05）,神经行为学评分显著增高（P<0.05）,HE染色、尼氏染色可见病灶区域明显减少,神经元损伤恢复效果良好（P<0.05）,血清SOD、MDA、LDH活力明显有所改善（P<0.05）,其中,蒙药萨乌日勒效果最显著,珍宝丸与赞丹-3效果仅次于萨乌日勒。结论 与模型组相比,经典蒙药萨乌日勒、珍宝丸、赞丹-3对小鼠脑缺血再灌注损伤均有显著改善作用,而相较于珍宝丸与赞丹-3,蒙药萨乌日勒改善脑缺血再灌注损伤效果更为显著,其抗脑缺血再灌注损伤作用可能是通过调控MAPK信号通路及抑制炎症反应发挥药理作用,但其具体活性成分及确切机制尚需进一步验证。

关键词: 萨乌日勒, 珍宝丸, 赞丹-3, 经典蒙药, 脑缺血再灌注损伤, 小鼠

Abstract: Objective To compare the protective effects of three classic Mongolian medicinal formulas—Saurile, Zhenbao pills, and Zandan-3 decoction—against cerebral ischemia-reperfusion injury in mice, and to identify the optimal formula for subsequent mechanistic investigations. Methods A total of 120 C57BL/6J mice were randomly divided into six groups: sham-operated group, model group, nimodi-pine group (46.8 mg·kg-1), Saurile group (338 mg·kg-1), Zhenbao pill group (780 mg·kg-1), and Zandan-3 decoction group (1 950 mg·kg-1), with 20 mice in each group. The treatment groups were administered with respective formulas via gavage for 10 days, while the sham-operated and model groups received an equivalent volume of saline. A transient middle cerebral artery occlusion model was established using the filament method, involving 1.5 hours of ischemia followed by 24 hours of reperfusion. The sham-operated group underwent the surgical procedure without filament insertion. After 24 hours of reperfusion, brain tissues were harvested for TTC staining to assess the ischemic area, HE and Nissl staining were used to observe pathological changes, and behavioral scoring was recorded. Serum samples were collected to measure levels of SOD, MDA, and LDH. The protective effects of Saurile, Zhenbao pills, and Zandan-3 decoction against cerebral ischemia-reperfusion injury were compared, and network pharmacology was employed to predict the targets of Saurile. Results Compared to the sham-operated group, there was a significant increase in the infarct area based on TTC staining, a markedly reduced neurobehavioral score (P<0.001), evident infarct lesions as evidenced by HE staining, and pronounced neuronal damage after Nissl staining in the model group (P<0.001). Serum levels of SOD, MDA, and LDH were significantly decreased (P<0.001). Compared with the model group, the nimodipine, Saurile, Zhenbao Pill, and Zandan-3 Decoction groups showed a significant decrease in the infarct area according to TTC staining (P<0.05), an increased neurobehavioral score (P<0.05), a decrease in lesions based on HE and Nissl staining (P<0.05), and higher serum levels of SOD, MDA, and LDH (P<0.05). Saurile proved to be the most effective, followed by Zhenbao pills and Zandan-3 decoction. Conclusion This study has found that, compared to the model group, the classic Mongolian medicinal formulas Saurile, Zhenbao pills, and Zandan-3 decoction can significantly ameliorate cerebral ischemia-reperfusion injury in mice. Saurile delivers the best effect, potentially by regulating the MAPK signaling pathway and inhibiting inflammatory responses. However, the specific active components and precise mechanisms require more study.

Key words: Saurile, Zhenbao Pill, Zandan-3, Classic Mongolian Medicine, Cerebral Ischemia-Reperfusion Injury, Mice

中图分类号: