中国药物警戒 ›› 2026, Vol. 23 ›› Issue (2): 127-133.
DOI: 10.19803/j.1672-8629.20250554

• 基础与临床研究 • 上一篇    下一篇

红参-制何首乌药对延缓SAMP8小鼠衰老过程及相关病理学改变研究

刘晶晶1, 徐蓓蕾2, 汪祺1#, 刘静1,*   

  1. 1中国食品药品检定研究院中药民族药检定所，北京 100050;
    2哈尔滨商业大学药学院，黑龙江 哈尔滨 150000
  • 收稿日期:2025-08-15 出版日期:2026-02-15 发布日期:2026-02-13
  • 通讯作者: *刘静，女，博士，研究员，中药质量控制研究。E-mail: liujing_zsm@126.com; #为共同通信作者。
  • 作者简介:刘晶晶，女，博士，助理研究员，中药质量控制研究。
  • 基金资助:
    国家自然科学基金资助项目 (82574563); 中国食品药品检定研究院中青年发展研究基金（2025A3）

Study on the Effect of Ginseng Radix et Rhizoma Rubra and Polygonum Multiflorum Radix Preparata Medicinal Pair on Delaying the Aging Process and Related Pathological Changes in SAMP8 Mice

LIU Jingjing1, XU Beilei2, WANG Qi1#, LIU Jing1,*   

  1. 1Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 100050, China;
    2School of Pharmacy, Harbin University of Commerce, Harbin Heilongjiang 150000, China
  • Received:2025-08-15 Online:2026-02-15 Published:2026-02-13

PDF (PC)

1

摘要： 目的 研究红参-制何首乌药对（RSSW）延缓快速老化小鼠（Senescence-Accelerated Mouse Prone 8，SAMP8）的衰老进程及对相关病理学改变的影响。方法 用6月龄的SAMP8小鼠及正常老化小鼠（Senescence Accelerated Mouse/Resistant 1，SAMR1）作为对照小鼠，小鼠分为5组，SAMR1对照组，SAMP8 模型组、SAMP8 +RSSW低剂量组（117 mg·kg-1，L-Dose）、SAMP8 + RSSW 高剂量组（234 mg·kg-1，H-Dose），多奈哌齐组（1.3 mg·kg-1），每组12只。连续灌胃给予RSSW 60 d处理。定期进行老化评分实验，采用新物体识别实验和疲劳转棒实验评估小鼠平衡运动能力。苏木精-伊红（HE）染色分析小鼠脏器组织形态学变化。ELISA分析脑脊液和海马中的Aβ沉积。采用RT-qPCR和Western Blot分析皮层组织中p21、p53 的mRNA和蛋白表达。对各组小鼠的体重和内脏指标进行分析，分析心脏、肺脏、肝脏、肾脏的抗氧化能力，包括SOD活性、GSH和MDA的含量，及抗炎能力包括IL-1β、IL-6和TNF-α的含量。采用流式细胞术检测免疫指标。结果 与对照小鼠相比，SAMP8小鼠的辨别指数和运动能力显著降低，RSSW给药可以显著增强其记忆能力和运动性能。RSSW可以有效逆转海马组织损伤，降低脑脊液中Aβ沉积，有效降低衰老标志物p21、p53的mRNA和蛋白表达，可以保护衰老导致的脏器损伤，改善免疫器官及脑萎缩，增加心脏、肺脏、肝脏、肾脏的抗氧化和抗炎能力，RSSW给药显著降低脾脏中CD4+/CD8+，从而改善机体的免疫功能，延缓机体老化进程。结论 RSSW可以用于改善老化引起的认知障碍，延缓衰老进程的病理改变。

关键词: 红参-制何首乌药对, 抗衰老, 抗氧化, 抗炎, 免疫, 小鼠

Abstract: Objective To investigate the effects of Ginseng Radix et Rhizoma Rubra and Polygonum Multiflorum Radix preparata medicinal pair (RSSW) formula on delaying aging and related pathological changes in senescence-accelerated prone 8 (SAMP8) mice. Methods Six-month-old SAMP8 mice and normal aging control mice (senescence accelerated mouse/resistant 1, SAMR1) were used. Mice were divided into five groups including the SAMR1 control group, the SAMP8 model group, the SAMP8 + RSSW low-dose group (117 mg·kg-1, L-Dose), the SAMP8+RSSW high-dose group (234 mg·kg-1, H-Dose), and the donepezil group (1.3 mg·kg-1). Each group consisted of 12 animals. RSSW was administered orally for 60 consecutive days. Aging scores were recorded while balance and motor abilities were evaluated using the novel object recognition and the rotarod tests. Hematoxylin and eosin (H&E) staining was performed to analyze the morphological changes in organ tissues. Enzyme-linked immunosorbent assay (ELISA) was used to measure Aβ deposition in the cerebrospinal fluid and hippocampus. RT-qPCR and Western blot analyses quantified p21, p53 mRNA and protein expressions in cortical tissues. Body weight and visceral parameters were recorded, including antioxidant capacity (superoxide dismutase [SOD] activity, glutathione [GSH], and malondialdehyde [MDA] contents) and anti-inflammatory markers (IL-1β, IL-6, and TNF-α levels) in the heart, liver, lung, and kidney of mice. Immune indexes were detected using flow cytometry. Results Compared with control mice, recognition indexes and motor ability were significantly reduced in SAMP8 mice but the administration of RSSW improved memory and motor performance. RSSW effectively reversed the damage to hippocampal tissues, reduced Aβ deposition in cerebrospinal fluid, decreased p21 and p53 mRNA and protein expressions, protected against organ damage, ameliorated atrophy of immune organs and brains, enhanced antioxidant and anti-inflammatory capacity in the heart, liver, lung and kidney, and lowered the CD4+/CD8+ ratio in the spleen, thereby improving immune function and delaying aging. Conclusion RSSW can improve cognitive impairment and delay pathological changes associated with aging.

Key words: Ginseng Radix et Rhizoma Rubra and Polygonum Multiflorum Radix preparata medicinal pair, Anti-aging, Antioxidant, Anti-inflammatory, Immunity, Mice

中图分类号: