中国药物警戒 ›› 2015, Vol. 12 ›› Issue (5): 261-263.

• 基础及临床研究 • 上一篇    下一篇

香加皮急性毒性与其杠柳毒苷含量相关性研究

徐鑫1,马志会2,3,孙达2,陈金堂2,周昆2*,刘洋2   

  1. 1.山西中医学院附属医院药剂科，山西太原 030024;
    2天津中医药大学中医药研究院方剂学教育部重点实验室，天津 300193;
    3山西晋城煤业集团总医院药剂科，山西晋城 048006
  • 收稿日期:2014-03-31 发布日期:2015-06-08
  • 通讯作者: 周昆，男，硕士，助理研究员，中药药理毒理学。
  • 作者简介:徐鑫，女，硕士，主管中药师，抗肿瘤临床药学。
  • 基金资助:
    “十一五”国家科技支撑计划重点项目（2006BAI14B01）；国家自然科学基金（81202850）。

Study on Correlation of the Content of Periplocin with Acute Toxicity of Cortex Periploce

XU Xin1 ,MA Zhi-hui2,3 ,SUN Da2 ,CHEN Jin-tang2 ,ZHOU Kun2 ,LIU Yang2   

  1. 1.Department of Pharmacy, Affiliated Hospital of Shanxi University of Traditional Chinese Medicine, Shanxi Taiyuan 030024, China;
    2.Institute of Traditional Chinese Medicine, Key Laboratory of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine,Tianjin 300193, China;
    3.Department of Pharmacy, General Hospital of Jincheng Anthracite Mining Group Co.Ltd, Shanxi Jincheng 048006, China
  • Received:2014-03-31 Published:2015-06-08

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摘要： 目的分析香加皮的急性毒性与其中杠柳毒苷含量的相关性。方法 HPLC测定 6批香加皮提取物中杠柳毒苷和 4-甲氧基水杨醛的含量；小鼠分别灌胃给予 6批香加皮提取物 32g膏 /kg，4-甲氧基水杨醛 200、250、300 mg·kg-1，观察 14天内的毒性反应及死亡情况。结果小鼠灌胃给药后 14天，香加皮 125.7g生药 /kg（含杠柳毒苷 159.552 mg·kg-1）， 106.6g生药 /kg（含杠柳毒苷 112.032 mg·kg-1），111.7g生药 /kg（含杠柳毒苷 91.584 mg·kg-1），123.8g生药 /kg（含杠柳毒苷 93.794 mg·kg-1），106.6g生药 /kg（含杠柳毒苷 46.784 mg·kg-1），122.6g生药 /kg（含杠柳毒苷 98.976 mg·kg-1）组死亡率分别为 70%、20%、0、0、0、20%，用杠柳毒苷的量与死亡率做回归考察相关性，相关系数 R2为 0.9678；4-甲氧基水杨醛 300 mg·kg-1组无死亡。结论柳毒苷含量与香加皮急性毒性实验结果有较好的相关性，杠柳毒苷含量的限定，可以较好的限制香加皮的毒性。

关键词: 香加皮, 杠柳毒苷, 4-甲氧基水杨醛, HPLC, 急性毒性, 相关性

Abstract: Objective To study the correlation of the content of periplocin with acute toxicity of in different places. Methods The content determination of periplocin and 4-methoxy salicylic aldehyde in Cortex Periploce was carried out by HPLC simultaneously. Mice were respectively orally administered with 32g·kg-1 of Cortex Periploce extract, 4-methoxy salicylic aldehyde 200, 250, 300 mg·kg-1, the toxic symptoms and the number of deaths were observed for fourteen days Results The acute toxicity study showed that the mortality in the groups of Cortex Periploce 125.7 g crude drug/kg(containing periplocin 159.552 mg·kg-1), 106.6g crude drug/kg (Containing Periplocin 112.032 mg·kg-1), 111.7g crude drug/kg (containing periplocin 91.584 mg·kg-1), 123.8g crude drug/kg (containing periplocin 93.794 mg·kg-1), 106.6 g crude drug/kg (containing periplocin 46.784 mg·kg-1), 122.6 g crude drug/kg (containing periplocin 98.976 mg·kg-1) were 70%, 20%,0,0,0 and 20% respectively. Linear regression correlation analysis showed that the correlation coefficient was 0.9678. The mortality in the group of 4-methoxy salicylic aldehyde 300 mg·kg-1 was 0 Conclusion There was good correlation between the content of periplocin and the results of the acute toxicity of Cortex Periploce , the toxicity response can be reduced by limiting the content of periplocin.

Key words: periplocin, 4-methoxy salicylic aldehyde, HPLC, acute toxicity, correlation

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