黄芩苷对人胃癌 SGC-7901细胞增殖抑制作用及其机制研究

摘要/Abstract

摘要： 目的探讨黄芩苷对人胃癌 SGC-7901细胞增殖抑制、诱导凋亡作用及其分子作用机制。方法 MTT法检测黄芩苷对 SGC-7901细胞的增殖抑制作用；PI单染法检测细胞周期；Annexin V/PI双染法检测细胞凋亡；Western blot法检测凋亡相关基因 Bcl-2和 Bax的蛋白表达水平。结果黄芩苷对 SGC-7901细胞生长有明显抑制作用，作用 24h和 48h对 SGC-7901细胞的半数抑制浓度（IC50）分别为 218.73 μmol·L-1，161.86 μmol·L-1，呈现时间—剂量依赖性； FCM结果显示黄芩苷能使 SGC-7901细胞阻滞在 G2/M期；Annexin V/PI双染法验证黄芩苷可以诱导细胞凋亡； Western blot结果显示 Bax蛋白水平明显上调，而 Bcl-2的蛋白表达水平降低。结论黄芩苷对 SGC-7901细胞增殖具有显著的抑制作用并可诱导细胞凋亡，其作用机制与其上调 Bax蛋白表达水平的同时，下调 Bcl-2蛋白表达水平，从而诱导细胞凋亡有关。

关键词: 黄芩苷, 胃癌, SGC-7901细胞, 增殖抑制, 机制

Abstract: Objective To investigate the inhibiting effect and its mechanism of baicalin on human gastric carcinoma SGC-7901 cells. Methods Growth inhibition rates of SGC-7901 cells were measured with MTT method, the cell cycle distribution was detected by PI single staining, apoptosis was detected by Annexin V/PI double staining, and the expression levels of Bcl-2, bax protein were analyzed by western blot. Results Baicalin inhibited the proliferation of SGC-7901 cells ina dose and time dependent manner. The IC50 of 24h and 48 h were 218.73 μmol·L-1 and 161.86μmol·L-1 respectively. The cell growth was arrested in G2/M phase. Fluorescent staining can detect the apoptotic cells induced by baicalin. The expression of Bax protein was increased while the Bcl-2 was decreased. Conclusion Baicalin not only inhibits proliferation but also induces apoptosis of SGC -7901 cells. Its mechanism is related to down -regulating Bcl-2 while up-regulating Bax.

Key words: baicalin, gastric carcinoma, SGC-7901 cells, proliferation inhibition, mechanism

中图分类号:

R965
R285.5

李峰,王根旺,钱彬彬,杨孙虎. 黄芩苷对人胃癌 SGC-7901细胞增殖抑制作用及其机制研究[J]. 中国药物警戒, 2015, 12(5): 257-260.

LI Feng, WANG Geng-wang, QIAN Bin-bin,YANG Sun-hu. Study on Inhibition Effect and Mechanism of Baicalin on Human Gastric Carcinoma SGC-7901 Cells[J]. Chinese Journal of Pharmacovigilance, 2015, 12(5): 257-260.

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